Collaborator Boster Bio
Primary AntibodyABMIUM Verified™Collaborator Tested
ABM-34319

Anti-Zebrafish LEO1 Antibody Picoband®

ABMIUM Verified™|Collaborator Tested|Boster Bio

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Anti-Zebrafish LEO1 Antibody Picoband® Boster Bio
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Anti-Zebrafish LEO1 Antibody Picoband® Boster Bio

Product Description

Anti-Zebrafish LEO1 Antibody Picoband® is a research-use antibody from Boster Bio for studying RNA polymerase-associated protein LEO1 in IF/ICC, IHC, IHC-P, WB workflows with listed reactivity for Zebrafish. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-Zebrafish LEO1 Antibody Picoband® catalog # AZQ6NYV9. Tested in WB, IHC, IF applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

RNA polymerase-associated protein LEO1 is an enzyme that in humans is encoded by the LEO1 gene. LEO1, parafibromin (CDC73; MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference AZQ6NYV9
Product category Primary Antibodies, Zebrafish Antibodies
Reactive species Zebrafish
Tested applications IF/ICC, IHC, IHC-P, WB
Available conjugations Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647
Gene name LEO1
Protein name RNA polymerase-associated protein LEO1
Immunogen E.coli-derived zebrafish LEO1 recombinant protein (Position: E333-K571).
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Purification Immunogen affinity purified.
Storage At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
UniProt Q6NYV9
NCBI Gene ID 404599

Available variants

Conjugation Size
Unconjugated 100 μg/vial
Biotin 100 μg/vial
Cy3 100 μg/vial
Fluoro488 100 μg/vial
Fluoro550 100 μg/vial
Fluoro594 100 μg/vial
FITC 100 μg/vial
HRP 100 μg/vial
APC 100 μg/vial
PE 100 μg/vial
Fluoro647 100 μg/vial

Key Facts

Product typePrimary Antibody Target: RNA polymerase-associated protein LEO1
Collaborator Boster Bio
Partner model AZQ6NYV9
Target RNA polymerase-associated protein LEO1
Reactivity Zebrafish
Applications IF/ICC,IHC,IHC-P,WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Dilution rangeWestern blot, 0.25-0.5 μg/ml, Zebrafish<br> Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Zebrafish<br> Immunofluorescence, 5 μg/ml, Zebrafish<br>
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Immunogen E.coli-derived zebrafish LEO1 recombinant protein (Position: E333-K571).
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Shelf life At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
AvailabilityAvailable
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Zebrafish

Specifications

Target
RNA polymerase-associated protein LEO1
Also known as
LEO1; RDL; RNA polymerase-associated protein LEO1; Replicative senescence down-regulated leo1-like protein
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Zebrafish
Tested applications
IF/ICCIHCIHC-PWB
Immunogen
E.coli-derived zebrafish LEO1 recombinant protein (Position: E333-K571).
Observed band
105 kDa
Dilution range
Western blot, 0.25-0.5 μg/ml, Zebrafish
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Immunofluorescence, 5 μg/ml, Zebrafish
Purification
Immunogen affinity purified.

Storage & Stability

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Storage buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Shelf life
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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