Collaborator Boster Bio
Primary AntibodyABMIUM Verified™Collaborator Tested
ABM-34931

Anti-Zebrafish GCLC Antibody Picoband®

ABMIUM Verified™|Collaborator Tested|Boster Bio

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Anti-Zebrafish GCLC Antibody Picoband® Boster Bio
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Anti-Zebrafish GCLC Antibody Picoband® Boster Bio

Product Description

Anti-Zebrafish GCLC Antibody Picoband® is a research-use antibody from Boster Bio for studying Glutamate--cysteine ligase in WB workflows with listed reactivity for Zebrafish. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-Zebrafish GCLC Antibody Picoband® catalog # AZQ6NV35. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Predicted to enable anion binding activity; glutamate-cysteine ligase activity; and magnesium ion binding activity. Predicted to be involved in several processes, including alpha-amino acid metabolic process; cell redox homeostasis; and glutathione biosynthetic process. Predicted to be part of glutamate-cysteine ligase complex. Is expressed in several structures, including alar plate midbrain region; gut; intermediate cell mass of mesoderm; midbrain; and sensory system. Human ortholog(s) of this gene implicated in congenital nonspherocytic hemolytic anemia 7; cystic fibrosis; hemolytic anemia; and myocardial infarction. Orthologous to human GCLC (glutamate-cysteine ligase catalytic subunit).

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference AZQ6NV35
Product category Primary Antibodies, Zebrafish Antibodies
Reactive species Zebrafish
Tested applications WB
Available conjugations Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647
Gene name GCLC
Protein name Glutamate--cysteine ligase
Immunogen E.coli-derived Zebrafish GCLC recombinant protein (Position: E13-K618).
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Purification Immunogen affinity purified.
Storage At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
UniProt Q6NV35
NCBI Gene ID 326857

Available variants

Conjugation Size
Unconjugated 100 μg/vial
Biotin 100 μg/vial
Cy3 100 μg/vial
Fluoro488 100 μg/vial
Fluoro550 100 μg/vial
Fluoro594 100 μg/vial
FITC 100 μg/vial
HRP 100 μg/vial
APC 100 μg/vial
PE 100 μg/vial
Fluoro647 100 μg/vial

Key Facts

Product typePrimary Antibody Target: Glutamate--cysteine ligase
Collaborator Boster Bio
Partner model AZQ6NV35
Target Glutamate--cysteine ligase
Reactivity Zebrafish
Applications WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Dilution rangeWestern blot, 0.25-0.5 μg/ml, Zebrafish<br>
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Immunogen E.coli-derived Zebrafish GCLC recombinant protein (Position: E13-K618).
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Shelf life At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
AvailabilityAvailable
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Zebrafish

Specifications

Target
Glutamate--cysteine ligase
Also known as
Gamma ECS; GCLC; GCS; GCS heavy chain; GLCL; GLCLC
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Zebrafish
Tested applications
WB
Immunogen
E.coli-derived Zebrafish GCLC recombinant protein (Position: E13-K618).
Observed band
80 kDa
Dilution range
Western blot, 0.25-0.5 μg/ml, Zebrafish
Purification
Immunogen affinity purified.

Storage & Stability

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Storage buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Shelf life
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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