ABM-34063
Anti-Zebrafish ETF1a/b Antibody Picoband®
ABMIUM Verified™|Collaborator Tested|Boster Bio
Primary Antibody supplied by Boster Bio Read more ›
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Product Description
Boster Bio Anti-Zebrafish-ETF1a-b-Antibody Picoband® catalog # AZA8KB65. Tested in WB, IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Eukaryotic translation termination factor 1 (eRF1),also known asTB3-1,is a protein that in humans is encoded by the ETF1 gene. It is mapped to 5q31.2. This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA,UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6,7,and X.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | AZA8KB65 |
| Product category | Primary Antibodies, Zebrafish Antibodies |
| Reactive species | Zebrafish |
| Tested applications | IHC, IHC-P, WB |
| Available conjugations | Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647 |
| Gene name | ETF1a/b |
| Protein name | Eukaryotic peptide chain release factor subunit 1 |
| Immunogen | E.coli-derived zebrafish ETF1a/b recombinant protein (Position: D9-K344). |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Purification | Immunogen affinity purified. |
| Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
| UniProt | A8KB65 |
| NCBI Gene ID | 563172 |
Available variants
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μg/vial |
| Biotin | 100 μg/vial |
| Cy3 | 100 μg/vial |
| Fluoro488 | 100 μg/vial |
| Fluoro550 | 100 μg/vial |
| Fluoro594 | 100 μg/vial |
| FITC | 100 μg/vial |
| HRP | 100 μg/vial |
| APC | 100 μg/vial |
| PE | 100 μg/vial |
| Fluoro647 | 100 μg/vial |
Key Facts
Product type
Primary Antibody›
Target: Eukaryotic peptide chain release factor subunit 1
| Target | Eukaryotic peptide chain release factor subunit 1 |
| Also known as | D5S1995; ERF; ERF1; ETF1; Eukaryotic release factor 1; Protein Cl1; RF1; SUP45L1; TB3 1 |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Species reactivity | Zebrafish |
| Form / buffer | Lyophilized |
| Shelf life | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647 |
| Tested applications |
IHCIHC-PWB |
| Dilution range | Western blot, 0.25-0.5 μg/ml, Zebrafish Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Zebrafish |
| Immunogen | E.coli-derived zebrafish ETF1a/b recombinant protein (Position: D9-K344). |
| Purification | Immunogen affinity purified. |
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Reactivity & Application Validation
1 species| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Zebrafish |
Specifications
Target
Eukaryotic peptide chain release factor subunit 1
Also known as
D5S1995; ERF; ERF1; ETF1; Eukaryotic release factor 1; Protein Cl1; RF1; SUP45L1; TB3 1
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Zebrafish
Tested applications
IHCIHC-PWB
Immunogen
E.coli-derived zebrafish ETF1a/b recombinant protein (Position: D9-K344).
Dilution range
Western blot, 0.25-0.5 μg/ml, Zebrafish
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Purification
Immunogen affinity purified.
Storage & Stability
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Long-term storage
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Storage conditions
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Storage buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Shelf life
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Compliance & Certifications
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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