Collaborator Boster Bio
Primary AntibodyABMIUM Verified™Collaborator Tested
ABM-34061

Anti-Zebrafish ENO1a Antibody Picoband®

ABMIUM Verified™|Collaborator Tested|Boster Bio

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Anti-Zebrafish ENO1a Antibody Picoband® Boster Bio
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Anti-Zebrafish ENO1a Antibody Picoband® Boster Bio

Product Description

Anti-Zebrafish ENO1a Antibody Picoband® is a research-use antibody from Boster Bio for studying phosphopyruvate hydratase in IHC, IHC-P, WB workflows with listed reactivity for Zebrafish. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-Zebrafish-ENO1a-Antibody Picoband® catalog # AZA0A2R8Q1X2. Tested in WB, IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Enolase 1 (ENO1) is a glycolytic enzyme expressed in most tissues. It is mapped to 1p36.23. This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference AZA0A2R8Q1X2
Product category Primary Antibodies, Zebrafish Antibodies
Reactive species Zebrafish
Tested applications IHC, IHC-P, WB
Available conjugations Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647
Gene name ENO1a
Protein name phosphopyruvate hydratase
Immunogen A synthetic peptide corresponding to a sequence in the middle region of zebrafish ENO1a.
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Purification Immunogen affinity purified.
Storage At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
UniProt A0A2R8Q1X2
NCBI Gene ID 334116

Available variants

Conjugation Size
Unconjugated 100 μg/vial
Biotin 100 μg/vial
Cy3 100 μg/vial
Fluoro488 100 μg/vial
Fluoro550 100 μg/vial
Fluoro594 100 μg/vial
FITC 100 μg/vial
HRP 100 μg/vial
APC 100 μg/vial
PE 100 μg/vial
Fluoro647 100 μg/vial

Key Facts

Product typePrimary Antibody Target: phosphopyruvate hydratase
Collaborator Boster Bio
Partner model AZA0A2R8Q1X2
Target phosphopyruvate hydratase
Reactivity Zebrafish
Applications IHC,IHC-P,WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Dilution rangeWestern blot, 0.25-0.5 μg/ml, Zebrafish<br> Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Zebrafish<br>
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Immunogen A synthetic peptide corresponding to a sequence in the middle region of zebrafish ENO1a.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Shelf life At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
AvailabilityAvailable
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Zebrafish

Specifications

Target
phosphopyruvate hydratase
Also known as
Alpha enolase; C myc promoter binding protein; ENO1; ENO1L1; Enolase 1; enolase 1; (alpha); MBP 1; MBPB1; MPB 1; MPB1; NNE; Non neural enolase; Phosphopyruvate hydratase; Plasminogen binding protein; PPH
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Zebrafish
Tested applications
IHCIHC-PWB
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of zebrafish ENO1a.
Dilution range
Western blot, 0.25-0.5 μg/ml, Zebrafish
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Purification
Immunogen affinity purified.

Storage & Stability

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Storage buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Shelf life
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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