Collaborator Boster Bio
Primary AntibodyABMIUM Verified™Collaborator Tested
ABM-34018

Anti-Zebrafish ATP6V1A Antibody Picoband®

ABMIUM Verified™|Collaborator Tested|Boster Bio

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Anti-Zebrafish ATP6V1A Antibody Picoband® Boster Bio
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Anti-Zebrafish ATP6V1A Antibody Picoband® Boster Bio

Product Description

Anti-Zebrafish ATP6V1A Antibody Picoband® is a research-use antibody from Boster Bio for studying H in IF/ICC, WB workflows with listed reactivity for Zebrafish. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-Zebrafish ATP6V1A Antibody Picoband® catalog # AZQ7SY46. Tested in WB, IF applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

V-type proton ATPase catalytic subunit A is an enzyme that in humans is encoded by the ATP6V1A gene. This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c, and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist.

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference AZQ7SY46
Product category Primary Antibodies, Zebrafish Antibodies
Reactive species Zebrafish
Tested applications IF/ICC, WB
Available conjugations Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647
Gene name ATP6V1A
Protein name H
Immunogen E.coli-derived zebrafish ATP6V1A recombinant protein (Position: A37-D617).
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Purification Immunogen affinity purified.
Storage At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
UniProt Q7SY46
NCBI Gene ID 394110

Available variants

Conjugation Size
Unconjugated 100 μg/vial
Biotin 100 μg/vial
Cy3 100 μg/vial
Fluoro488 100 μg/vial
Fluoro550 100 μg/vial
Fluoro594 100 μg/vial
FITC 100 μg/vial
HRP 100 μg/vial
APC 100 μg/vial
PE 100 μg/vial
Fluoro647 100 μg/vial

Key Facts

Product typePrimary Antibody Target: H
Collaborator Boster Bio
Partner model AZQ7SY46
Target H
Reactivity Zebrafish
Applications IF/ICC,WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Dilution rangeWestern blot, 0.25-0.5 μg/ml, Zebrafish<br> Immunofluorescence, 5 μg/ml, Zebrafish<br>
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Immunogen E.coli-derived zebrafish ATP6V1A recombinant protein (Position: A37-D617).
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Shelf life At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
AvailabilityAvailable
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Zebrafish

Specifications

Target
H
Also known as
ATP6A1; ATP6V1A; ATP6V1A1; HO68; V ATPase 69 kDa subunit; V ATPase subunit A; VA68; Vacuolar ATPase isoform VA68; Vma1; VPP2
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Zebrafish
Tested applications
IF/ICCWB
Immunogen
E.coli-derived zebrafish ATP6V1A recombinant protein (Position: A37-D617).
Dilution range
Western blot, 0.25-0.5 μg/ml, Zebrafish
Immunofluorescence, 5 μg/ml, Zebrafish
Purification
Immunogen affinity purified.

Storage & Stability

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Storage buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Shelf life
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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