Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-21576

Anti-Werner's syndrome helicase WRN/WRN Antibody Picoband®

ABMIUM Verified™|Collaborator Tested|Boster Bio

Primary Antibody supplied by Boster Bio Read more ›

Anti-Werner's syndrome helicase WRN/WRN Antibody Picoband® Boster Bio
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Anti-Werner's syndrome helicase WRN/WRN Antibody Picoband® Boster Bio

Product Description

Anti-Werner's syndrome helicase WRN/WRN Antibody Picoband® is a research-use antibody from Boster Bio for studying Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN in ELISA, Flow Cytometry, IHC, IHC-P, WB workflows with listed reactivity for Human, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-Werner's syndrome helicase WRN/WRN Antibody Picoband® catalog # PB10107. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Werner syndrome ATP-dependent helicase, also known as DNA helicase, RecQ-like type 3, is an enzyme that in humans is encoded by the WRN gene. This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging.

Product information

Key Facts

Product typePrimary Antibodies Target: Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN
Collaborator Boster Bio
Partner model PB10107
Target Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN
Reactivity Human,Rat
Applications ELISA,Flow Cytometry,IHC,IHC-P,WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Cross-reactivityNo cross-reactivity with other proteins.
Dilution rangeWestern blot, 0.1-0.5μg/ml, Human, Rat<br>
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Immunogen E. coli-derived human WRN recombinant protein (Position: Q122-N240). Human WRN shares 84% amino acid (aa) sequence identity with mouse WRN.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Purity No cross reactivity with other proteins.
Shelf life Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
AvailabilityAvailable
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human
Rat

Specifications

Target
Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN
Also known as
WRN RecQ like helicase; RECQ3; RECQL2; RECQL3
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Human,Rat
Tested applications
ELISAFlow CytometryIHCIHC-PWB
Immunogen
E. coli-derived human WRN recombinant protein (Position: Q122-N240). Human WRN shares 84% amino acid (aa) sequence identity with mouse WRN.
Observed band
162 kDa, 200 kDa
Dilution range
Western blot, 0.1-0.5μg/ml, Human, Rat
Purification
Immunogen affinity purified.

Storage & Stability

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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