Primary Antibody supplied by Boster Bio Read more ›
Anti-VPRBP/DCAF1 Antibody Picoband® is a research-use antibody from Boster Bio for studying DDB1- and CUL4-associated factor 1 in ELISA, Flow Cytometry, IF/ICC, IHC, IHC-P, WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Boster Bio Anti-VPRBP/DCAF1 Antibody Picoband® catalog # A31776. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.VprBP was first identified as a protein that can interact with HIV-1 viral protein R. It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein. VprBP is a 1,507-amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells. Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway.
| Collaborator | Boster Bio |
| Partner model | A31776 |
| Target | DDB1- and CUL4-associated factor 1 |
| Reactivity | Human,Mouse,Rat |
| Applications | ELISA,Flow Cytometry,IF/ICC,IHC,IHC-P,WB |
| Conjugation | Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647 |
| Cross-reactivity | No cross-reactivity with other proteins. |
| Dilution range | Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5μg/ml, Human<br> Flow Cytometry (Fixed), 1-3μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5μg/ml, -<br> |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived human VPRBP/DCAF1 recombinant protein (Position: M1-R195). |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Purification | Immunogen affinity purified. |
| Shelf life | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| Availability | Available |
| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | Flow Cytometry | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Human | ||||||||
| Mouse | ||||||||
| Rat |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
For research applications. Not for diagnostic use.
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