Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-23116

Anti-USP25 Antibody Picoband®

ABMIUM Verified™|Collaborator Tested|Boster Bio

Primary Antibody supplied by Boster Bio Read more ›

Anti-USP25 Antibody Picoband® Boster Bio
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Anti-USP25 Antibody Picoband® Boster Bio

Product Description

Anti-USP25 Antibody Picoband® is a research-use antibody from Boster Bio for studying Ubiquitin carboxyl-terminal hydrolase 25 in ELISA, Flow Cytometry, IF/ICC, IHC, IHC-P, WB workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-USP25 Antibody Picoband® catalog # A06182-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Ubiquitin specific peptidase 25 is a protein that in humans is encoded by the USP25 gene. It is mapped to 21q21.1. Ubiquitin is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25.

Product information

Key Facts

Product typePrimary Antibodies Target: Ubiquitin carboxyl-terminal hydrolase 25
Collaborator Boster Bio
Partner model A06182-1
Target Ubiquitin carboxyl-terminal hydrolase 25
Reactivity Human,Mouse,Rat,Monkey
Applications ELISA,Flow Cytometry,IF/ICC,IHC,IHC-P,WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Cross-reactivityNo cross-reactivity with other proteins.
Dilution rangeWestern blot, 0.1-0.25μg/ml, Human, Mouse, Rat, Monkey<br> Immunocytochemistry/Immunofluorescence, 2μg/ml, Human<br> Flow Cytometry (Fixed), 1-3μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5μg/ml, -<br>
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Immunogen E.coli-derived human USP25 recombinant protein (Position: M1-D1053).
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Purity No cross reactivity with other proteins.
Shelf life Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
AvailabilityAvailable
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Application Validation Matrix

4 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human
Mouse
Rat
Monkey

Specifications

Target
Ubiquitin carboxyl-terminal hydrolase 25
Also known as
Deubiquitinating enzyme 25; Ubiquitin thiolesterase 25; USP on chromosome 21; USP21; USP25
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Human,Mouse,Rat,Monkey
Tested applications
ELISAFlow CytometryIF/ICCIHCIHC-PWB
Immunogen
E.coli-derived human USP25 recombinant protein (Position: M1-D1053).
Observed band
125 kDa
Dilution range
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat, Monkey
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Purification
Immunogen affinity purified.

Storage & Stability

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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