ABM-29481
Anti-TRIM25 Monoclonal Antibody
ABMIUM Verified™|Collaborator Tested|Boster Bio
Primary Antibody supplied by Boster Bio Read more ›
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Product Description
Boster Bio Anti-TRIM25 Monoclonal Antibody catalog # M03232. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | M03232 |
| Product category | Primary Antibodies, Rabbit Monoclonal Antibodies |
| Reactive species | Human, Mouse, Rat |
| Tested applications | Flow Cytometry, IF/ICC, IHC, IP, WB |
| Available conjugations | Unconjugated |
| Gene name | TRIM25 |
| Protein name | E3 ubiquitin/ISG15 ligase TRIM25 |
| Immunogen | A synthesized peptide derived from human TRIM25 Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Purification | Affinity-chromatography |
| Storage | Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles. |
| UniProt | Q14258 |
| NCBI Gene ID | 7706 |
Available variants
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μl/vial |
Key Facts
Product type
Primary Antibody›
Target: E3 ubiquitin/ISG15 ligase TRIM25
| Target | E3 ubiquitin/ISG15 ligase TRIM25 |
| Also known as | EFP; RING finger protein 147; RNF147; TRIM25; tripartite motif containing 25; Z147; Zinc finger protein 147; ZNF147 |
| Concentration | 0.5mg/ml |
| Species reactivity | Human,Mouse,Rat |
| Observed band | 71 kDa |
| Form / buffer | Liquid |
| Shelf life | Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles. |
| Host species | Rabbit |
| Clonality | Monoclonal |
| Clone number | Clone: ADFG-20 |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Tested applications |
Flow CytometryIF/ICCIHCIPWB |
| Dilution range | WB 1:1000-5000 IHC 1:50-200 ICC/IF 1:50-200 IP 1:50 FC 1:60 |
| Immunogen | A synthesized peptide derived from human TRIM25 Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. |
| Purification | Affinity-chromatography |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Human | ||||||||
| Mouse | ||||||||
| Rat |
Specifications
Target
E3 ubiquitin/ISG15 ligase TRIM25
Also known as
EFP; RING finger protein 147; RNF147; TRIM25; tripartite motif containing 25; Z147; Zinc finger protein 147; ZNF147
Concentration
0.5mg/ml
Form / buffer
Liquid
Host species
Rabbit
Clonality
Monoclonal
Clone number
Clone: ADFG-20
Isotype
Rabbit IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
Flow CytometryIF/ICCIHCIPWB
Immunogen
A synthesized peptide derived from human TRIM25 Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection.
Observed band
71 kDa
Dilution range
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:60
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:60
Purification
Affinity-chromatography
Storage & Stability
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Storage buffer
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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