Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-24504

Anti-SHC Antibody Picoband® (monoclonal, 6D6E1

ABMIUM Verified™|Collaborator Tested|Boster Bio

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Anti-SHC Antibody Picoband® (monoclonal, 6D6E1 Boster Bio
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Anti-SHC Antibody Picoband® (monoclonal, 6D6E1 Boster Bio

Product Description

Anti-SHC Antibody Picoband® (monoclonal, 6D6E1 is a research-use antibody from Boster Bio for studying SHC-transforming protein 1 in ELISA, Flow Cytometry, IHC, IHC-P, WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-SHC Antibody Picoband® (monoclonal, 6D6E1) catalog # M00796-3. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

SHC, also known as SHC1 (SHC-transforming protein 1) or SHCA, is a protein that in humans is encoded by the SHC1 gene. SCOP classifies the 3D structure as belonging to the SH2 domain family. This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.

Product information

Key Facts

Product typePrimary Antibodies Target: SHC-transforming protein 1
Collaborator Boster Bio
Partner model M00796-3
Target SHC-transforming protein 1
Reactivity Human,Mouse,Rat
Applications ELISA,Flow Cytometry,IHC,IHC-P,WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Cross-reactivityNo cross-reactivity with other proteins.
Dilution rangeWestern blot, 0.25-0.5 μg/ml, Human, Mouse, Rat<br>
Host Mouse
Clonality Monoclonal
Clone Clone: 6D6E1
Isotype Mouse IgG2b
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human SHC, identical to the related mouse and rat sequences.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Shelf life At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
AvailabilityAvailable
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human
Mouse
Rat

Specifications

Target
SHC-transforming protein 1
Also known as
SH2 domain protein C1; SHC; SHC transforming protein 1; SHC transforming protein 3; SHC transforming protein A; SHC1; SHCA
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form / buffer
Lyophilized
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: 6D6E1
Isotype
Mouse IgG2b
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Human,Mouse,Rat
Tested applications
ELISAFlow CytometryIHCIHC-PWB
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SHC, identical to the related mouse and rat sequences.
Observed band
65 kDa
Dilution range
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Purification
Immunogen affinity purified.

Storage & Stability

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Storage buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Shelf life
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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