Collaborator Boster Bio
Primary AntibodyABMIUM Verified™Collaborator Tested
ABM-29534

Anti-SAE2 / UBA2 Monoclonal Antibody

ABMIUM Verified™|Collaborator Tested|Boster Bio

Primary Antibody supplied by Boster Bio Read more ›

Anti-SAE2 / UBA2 Monoclonal Antibody Boster Bio
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Anti-SAE2 / UBA2 Monoclonal Antibody Boster Bio

Product Description

Anti-SAE2 / UBA2 Monoclonal Antibody is a research-use antibody from Boster Bio for studying SUMO-activating enzyme subunit 2 in IF/ICC, IHC, IP, WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-SAE2 / UBA2 Monoclonal Antibody catalog # M03816. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference M03816
Product category Primary Antibodies, Rabbit Monoclonal Antibodies
Reactive species Human, Mouse, Rat
Tested applications IF/ICC, IHC, IP, WB
Available conjugations Unconjugated
Gene name UBA2
Protein name SUMO-activating enzyme subunit 2
Immunogen A synthesized peptide derived from human SAE2 / UBA2 The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Host Rabbit
Clonality Monoclonal
Isotype Rabbit IgG
Purification Affinity-chromatography
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
UniProt Q9UBT2
NCBI Gene ID 10054

Available variants

Conjugation Size
Unconjugated 100 μl/vial

Key Facts

Product typePrimary Antibody Target: SUMO-activating enzyme subunit 2
Collaborator Boster Bio
Partner model M03816
Target SUMO-activating enzyme subunit 2
Reactivity Human,Mouse,Rat
Applications IF/ICC,IHC,IP,WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000<br>IHC 1:50-200<br>ICC/IF 1:50-200<br>IP 1:50<br>
Host Rabbit
Clonality Monoclonal
Clone Clone: AEAI-21
Isotype Rabbit IgG
Immunogen A synthesized peptide derived from human SAE2 / UBA2 The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Concentration 0.5mg/ml
Purification Affinity-chromatography
Shelf life Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
AvailabilityAvailable
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human
Mouse
Rat

Specifications

Target
SUMO-activating enzyme subunit 2
Also known as
ARX; HRIHFB2115; SAE2; UBA2; UBLE1B
Concentration
0.5mg/ml
Form / buffer
Liquid
Host species
Rabbit
Clonality
Monoclonal
Clone number
Clone: AEAI-21
Isotype
Rabbit IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
IF/ICCIHCIPWB
Immunogen
A synthesized peptide derived from human SAE2 / UBA2 The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Observed band
43 kDa
Dilution range
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
Purification
Affinity-chromatography

Storage & Stability

Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Storage buffer
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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