Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-23522

Anti-PROM1/CD133 Antibody Picoband® (monoclonal, 7F13

ABMIUM Verified™|Collaborator Tested|Boster Bio

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Anti-PROM1/CD133 Antibody Picoband® (monoclonal, 7F13 Boster Bio
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Anti-PROM1/CD133 Antibody Picoband® (monoclonal, 7F13 Boster Bio

Product Description

Anti-PROM1/CD133 Antibody Picoband® (monoclonal, 7F13 is a research-use antibody from Boster Bio for studying Prominin-1 in ELISA, Flow Cytometry, IHC, IHC-P, WB workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-PROM1/CD133 Antibody Picoband® (monoclonal, 7F13) catalog # M01767-4. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Prominin-1, also known as CD133, is a glycoprotein that in humans is encoded by the PROM1 gene. It is mapped to 4p15.32. Prominin-1 is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localize to cellular protrusions. This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. It has been proposed to act as an organizer of cell membrane topology. Prominin-1 was expressed not only on metastatic colon cancer cells, but also on differentiated colonic epithelium in both adult mice and humans.

Product information

Key Facts

Product typePrimary Antibodies Target: Prominin-1
Collaborator Boster Bio
Partner model M01767-4
Target Prominin-1
Reactivity Human
Applications ELISA,Flow Cytometry,IHC,IHC-P,WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Cross-reactivityNo cross-reactivity with other proteins.
Dilution rangeWestern blot, 0.25-0.5μg/ml, Human<br>
Host Mouse
Clonality Monoclonal
Clone Clone: 7F13
Isotype Mouse IgG1
Immunogen E.coli-derived human PROM1/CD133 recombinant protein (Position: P531-H865). HumanPROM1/CD133 shares 61% amino acid (aa) sequence identity with mouse PROM1/CD133.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Shelf life Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
AvailabilityAvailable
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human

Specifications

Target
Prominin-1
Also known as
AC133; Antigen AC133; CD133; CORD12; MCDR2; MSTP061; PROM1; PROM1 1; PROM1 1; 2; 3; PROM1 1; 2; 3; 5; 7; PROM1 1; 6; 7; PROM1 2; PROM1 2; 3; 4; 5; prominin 1; Prominin like protein 1; PROML1; RP41; STGD4
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form / buffer
Lyophilized
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: 7F13
Isotype
Mouse IgG1
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Human
Tested applications
ELISAFlow CytometryIHCIHC-PWB
Immunogen
E.coli-derived human PROM1/CD133 recombinant protein (Position: P531-H865). HumanPROM1/CD133 shares 61% amino acid (aa) sequence identity with mouse PROM1/CD133.
Observed band
120 kDa
Dilution range
Western blot, 0.25-0.5μg/ml, Human
Purification
Immunogen affinity purified.

Storage & Stability

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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