Primary Antibody supplied by Boster Bio Read more ›
Anti-POLR1E Antibody Picoband® is a research-use antibody from Boster Bio for studying DNA-directed RNA polymerase I subunit RPA49 in ELISA, Flow Cytometry, IF/ICC, IHC, IHC-P, IP, WB workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.
Boster Bio Anti-POLR1E Antibody Picoband® catalog # A11077-2. Tested in WB, ICC/IF, IP, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.Predicted to enable DNA binding activity and RNA polymerase I general transcription initiation factor binding activity. Involved in nucleolar large rRNA transcription by RNA polymerase I and transcription initiation at RNA polymerase I promoter. Located in fibrillar center and nucleoplasm. Part of RNA polymerase I complex.
| Collaborator | Boster Bio |
| Partner model | A11077-2 |
| Target | DNA-directed RNA polymerase I subunit RPA49 |
| Reactivity | Human |
| Applications | ELISA,Flow Cytometry,IF/ICC,IHC,IHC-P,IP,WB |
| Conjugation | Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647 |
| Cross-reactivity | No cross-reactivity with other proteins |
| Dilution range | Western blot, 0.25-0.5 μg/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> Immunoprecipitation, 2-4 μg/ml, Human<br> ELISA, 0.1-0.5 μg/ml<br> |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived human POLR1E recombinant protein (Position: M1-Q291). Human POLR1E shares 78.9% amino acid (aa) sequence identity with mouse POLR1E. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Purification | Immunogen affinity purified. |
| Shelf life | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
| Availability | Available |
| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | Flow Cytometry | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Human |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
For research applications. Not for diagnostic use.
No customer reviews yet.
