Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-27091

Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175)

ABMIUM Verified™|Collaborator Tested|Boster Bio

Primary Antibody supplied by Boster Bio Read more ›

Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175) Boster Bio
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Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175) Boster Bio

Product Description

Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175) is a research-use antibody from Boster Bio for studying RAC-alpha serine/threonine-protein kinase in WB workflows with listed reactivity for Chicken, Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175) catalog # MA1085. Tested in WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.

PKB alpha also knows as V-AKT murine thymoma vial oncogene homolog 1 (ATK1). AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and is abrogated by mutations in the pleckstrin homology domain of AKT1. AKT1 gene is mapped to chromosome 14q32.3. Akt1/protein kinase B-alpha is critical for ischemic and VEGF-mediated angiogenesis. Akt1 regulates pathological angiogenesis, vascular maturation and permeability in vivo.

Product information

Key Facts

Product typePrimary Antibodies Target: RAC-alpha serine/threonine-protein kinase
Collaborator Boster Bio
Partner model MA1085
Target RAC-alpha serine/threonine-protein kinase
Reactivity Chicken,Human,Mouse,Rat
Applications WB
ConjugationUnconjugated
Cross-reactivityNo cross-reactivity with other proteins
Dilution rangeWestern blot, 0.25-0.5μg/ml, Human, mouse, rat, chicken<br>
Host Mouse
Clonality Monoclonal
Clone Clone: PKB-175
Isotype Mouse IgG1
Immunogen Synthetic peptide corresponding to amino acids 461-477 of human PKB alpha/Akt1, conjugated to KLH.
Concentration Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Purification Ascites
Purity No cross reactivity with other proteins.
Shelf life Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
AvailabilityAvailable
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Application Validation Matrix

4 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Chicken
Human
Mouse
Rat

Specifications

Target
RAC-alpha serine/threonine-protein kinase
Also known as
AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA; ONCOGENE AKT1; PROTEIN KINASE B-ALPHA; RAC SERINE/THREONINE PROTEIN KINASE; Protein kinase B; Proto-oncogene c-Akt; RAC-PK-alpha
Concentration
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: PKB-175
Isotype
Mouse IgG1
Conjugation
Unconjugated
Species reactivity
Chicken,Human,Mouse,Rat
Tested applications
WB
Immunogen
Synthetic peptide corresponding to amino acids 461-477 of human PKB alpha/Akt1, conjugated to KLH.
Observed band
60 kDa
Dilution range
Western blot, 0.25-0.5μg/ml, Human, mouse, rat, chicken
Purification
Ascites

Storage & Stability

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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