ABM-27091
Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175)
ABMIUM Verified™|Collaborator Tested|Boster Bio
Primary Antibody supplied by Boster Bio Read more ›
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Product Description
Boster Bio Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175) catalog # MA1085. Tested in WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.
PKB alpha also knows as V-AKT murine thymoma vial oncogene homolog 1 (ATK1). AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and is abrogated by mutations in the pleckstrin homology domain of AKT1. AKT1 gene is mapped to chromosome 14q32.3. Akt1/protein kinase B-alpha is critical for ischemic and VEGF-mediated angiogenesis. Akt1 regulates pathological angiogenesis, vascular maturation and permeability in vivo.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | MA1085 |
| Product category | Primary Antibodies, Mouse Monoclonal Antibodies |
| Reactive species | Chicken, Human, Mouse, Rat |
| Tested applications | WB |
| Available conjugations | Unconjugated |
| Gene name | AKT1 |
| Protein name | RAC-alpha serine/threonine-protein kinase |
| Specificity | No cross reactivity with other proteins. |
| Immunogen | Synthetic peptide corresponding to amino acids 461-477 of human PKB alpha/Akt1, conjugated to KLH. |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Purification | Ascites |
| Storage | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| UniProt | P47196 |
| NCBI Gene ID | Human(207), Mouse(11651), Rat(24185) |
Available variants
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μg/vial |
Key Facts
Product type
Primary Antibody›
Target: RAC-alpha serine/threonine-protein kinase
| Target | RAC-alpha serine/threonine-protein kinase |
| Also known as | AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA; ONCOGENE AKT1; PROTEIN KINASE B-ALPHA; RAC SERINE/THREONINE PROTEIN KINASE; Protein kinase B; Proto-oncogene c-Akt; RAC-PK-alpha |
| Concentration | Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml. |
| Species reactivity | Chicken,Human,Mouse,Rat |
| Observed band | 60 kDa |
| Purity | No cross reactivity with other proteins. |
| Form / buffer | Lyophilized |
| Shelf life | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| Host species | Mouse |
| Clonality | Monoclonal |
| Clone number | Clone: PKB-175 |
| Isotype | Mouse IgG1 |
| Conjugation | Unconjugated |
| Non-cross-reactive with | No cross-reactivity with other proteins |
| Tested applications |
WB |
| Dilution range | Western blot, 0.25-0.5μg/ml, Human, mouse, rat, chicken |
| Immunogen | Synthetic peptide corresponding to amino acids 461-477 of human PKB alpha/Akt1, conjugated to KLH. |
| Purification | Ascites |
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Reactivity & Application Validation
4 speciess| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Chicken | ||||||||
| Human | ||||||||
| Mouse | ||||||||
| Rat |
Specifications
Target
RAC-alpha serine/threonine-protein kinase
Also known as
AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA; ONCOGENE AKT1; PROTEIN KINASE B-ALPHA; RAC SERINE/THREONINE PROTEIN KINASE; Protein kinase B; Proto-oncogene c-Akt; RAC-PK-alpha
Concentration
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: PKB-175
Isotype
Mouse IgG1
Conjugation
Unconjugated
Species reactivity
Chicken,Human,Mouse,Rat
Tested applications
WB
Immunogen
Synthetic peptide corresponding to amino acids 461-477 of human PKB alpha/Akt1, conjugated to KLH.
Observed band
60 kDa
Dilution range
Western blot, 0.25-0.5μg/ml, Human, mouse, rat, chicken
Purification
Ascites
Storage & Stability
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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