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Anti-PIK3R4 Antibody Picoband® is a research-use antibody from Boster Bio for studying Phosphoinositide 3-kinase regulatory subunit 4 in ELISA, Flow Cytometry, IHC, IHC-P, WB workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.
Boster Bio Anti-PIK3R4 Antibody Picoband® catalog # A06618-1. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.Phosphoinositide 3-kinase regulatory subunit 4, also known as PI3-kinase regulatory subunit 4 or PI3-kinase p150 subunit or phosphoinositide 3-kinase adaptor protein, or VPS15 is an enzyme that in humans is encoded by the PIK3R4 gene. The serine/threonine PI3 kinase regulatory subunit 4 (PIK3R4, Vps15) is the mammalian homologue of the yeast vacuolar protein sorting 15. PIK3R4 regulates the kinase activity of PI3K class III and anchors the kinase to cellular membranes through myristoylation. Recruitment of PI3K class III to the site of early endosome fusion and docking is ly mediated by PIK3R4 binding to the small GTPase Rab5 through its HEAT and WD-40 domains. The PIK3R4/PI3K class III plays a role in late endosome function through PIK3R4 binding to the Rab7 GTPase. In addition to its role in trafficking, the PIK3R4/PI3K class III complex interacts with beclin-1 to play a role during several stages of autophagy. Autophagosome formation is stimulated when Atg14 complexes with PIK3R4, PI3K class III, and beclin-1. The UVRAG protein competes with Atg14 for beclin-1 binding, forming a mutually exclusive complex with PIK3R4, PI3K class III, and beclin-1 that regulates autophagosome maturation. Autophagosome maturation is impaired in the presence of the beclin-1-binding protein Rubicon. Co-expression of PIK3R4 is required for PI3K class III activation and regulation by both beclin-1/UVRAG and by nutrients. Overexpression of PIK3R4 protein has been associated with decreased survival in patients with ovarian tumors, while mutations of the corresponding PIK3R4 gene are associated with metastatic melanoma, suggesting that PIK3R4 functions in cancer.
| Collaborator | Boster Bio |
| Partner model | A06618-1 |
| Target | Phosphoinositide 3-kinase regulatory subunit 4 |
| Reactivity | Human |
| Applications | ELISA,Flow Cytometry,IHC,IHC-P,WB |
| Conjugation | Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647 |
| Cross-reactivity | No cross reactivity with other proteins. |
| Dilution range | Western blot, 0.25-0.5 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br> |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | E.coli-derived human PIK3R4 recombinant protein (Position: R36-Q1339). Human PIK3R4 shares 95.7% and 95.6% amino acid (aa) sequence identity with mouse and rat PIK3R4, respectively. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Purification | Immunogen affinity purified. |
| Shelf life | At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing. |
| Availability | Available |
| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | Flow Cytometry | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Human |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
For research applications. Not for diagnostic use.
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