ABM-29280
Anti-Phospho-Histone H3 (T3) H3F3A Monoclonal Antibody
ABMIUM Verified™|Collaborator Tested|Boster Bio
Primary Antibody supplied by Boster Bio Read more ›
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Product Description
Boster Bio Anti-Phospho-Histone H3 (T3) H3F3A Monoclonal Antibody catalog # MP06819. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | MP06819 |
| Product category | Primary Antibodies, Rabbit Monoclonal Antibodies |
| Reactive species | Human |
| Tested applications | Flow Cytometry, IF/ICC, IHC, WB |
| Available conjugations | Unconjugated |
| Gene name | H3F3A |
| Protein name | Histone H3.3 |
| Immunogen | A synthesized peptide derived from human Phospho-Histone H3 (T3) H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Purification | Affinity-chromatography |
| Storage | Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles. |
| UniProt | P84243 |
| NCBI Gene ID | 3020 |
Available variants
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μl/vial |
Key Facts
Product type
Primary Antibody›
Target: Histone H3.3
| Target | Histone H3.3 |
| Also known as | H3 histone; family 3A; H3.3A; H3.3B; H3F3; H3F3A; H3F3B; Histone H3.3 |
| Concentration | 0.5mg/ml |
| Species reactivity | Human |
| Observed band | 15 kDa |
| Form / buffer | Liquid |
| Shelf life | Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles. |
| Host species | Rabbit |
| Clonality | Monoclonal |
| Clone number | Clone: AEO-8 |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Tested applications |
Flow CytometryIF/ICCIHCWB |
| Dilution range | WB 1:500-2000 IHC 1:50-200 ICC/IF 1:50-200 FC 1:50 |
| Immunogen | A synthesized peptide derived from human Phospho-Histone H3 (T3) H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Purification | Affinity-chromatography |
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Reactivity & Application Validation
1 species| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Human |
Specifications
Target
Histone H3.3
Also known as
H3 histone; family 3A; H3.3A; H3.3B; H3F3; H3F3A; H3F3B; Histone H3.3
Concentration
0.5mg/ml
Form / buffer
Liquid
Host species
Rabbit
Clonality
Monoclonal
Clone number
Clone: AEO-8
Isotype
Rabbit IgG
Conjugation
Unconjugated
Species reactivity
Human
Tested applications
Flow CytometryIF/ICCIHCWB
Immunogen
A synthesized peptide derived from human Phospho-Histone H3 (T3) H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Observed band
15 kDa
Dilution range
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Purification
Affinity-chromatography
Storage & Stability
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Storage buffer
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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