ABM-26109
Anti-PHF15/JADE2 Antibody Picoband®
ABMIUM Verified™|Collaborator Tested|Boster Bio
Primary Antibody supplied by Boster Bio Read more ›
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Product Description
Boster Bio Anti-PHF15/JADE2 Antibody Picoband® catalog # A12993-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
PHF15, also known as Protein Jade-2, has 3 isoforms, a 790 amino acid isoform that is 87 kDa, a 789 amino acid isoform that is 87 kDa and a 562 amino acid isoform that is 63 kDa, has a histone H4-specific acetyltransferase activity as a component of the HBO1complex, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. This protein has been shown an interaction with ING4, KAT7, ING5, PACSIN, ZBTB3, ZBTB5, ZNF496, and VHL proteins in histone H3 acetylation, histone H4-K5 acetylation, histone H4-K8 acetylation, and histone H4-K12 acetylation biological processes.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | A12993-1 |
| Product category | Primary Antibodies |
| Reactive species | Human |
| Tested applications | ELISA, Flow Cytometry, IHC, IHC-P, WB |
| Available conjugations | Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647 |
| Gene name | JADE2 |
| Protein name | E3 ubiquitin-protein ligase Jade-2 |
| Immunogen | E.coli-derived mouse Cd14 recombinant protein (Position: D36-A260). Mouse Cd14 shares 68.2% and 85.7% amino acid (aa) sequence identity with human and rat CD14, respectively. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Immunogen affinity purified. |
| Storage | At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing. |
| UniProt | Q9NQC1 |
| NCBI Gene ID | 23338 |
Available variants
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μg/vial |
| Biotin | 100 μg/vial |
| Cy3 | 100 μg/vial |
| Fluoro488 | 100 μg/vial |
| Fluoro550 | 100 μg/vial |
| Fluoro594 | 100 μg/vial |
| FITC | 100 μg/vial |
| HRP | 100 μg/vial |
| APC | 100 μg/vial |
| PE | 100 μg/vial |
| Fluoro647 | 100 μg/vial |
Key Facts
Product type
Primary Antibody›
Target: E3 ubiquitin-protein ligase Jade-2
| Target | E3 ubiquitin-protein ligase Jade-2 |
| Also known as | JADE2; KIAA0239; PHF15; E3 ubiquitin-protein ligase Jade-2; Jade family PHD finger protein 2; PHD finger protein 15 |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Species reactivity | Human |
| Observed band | 120 kDa |
| Form / buffer | Lyophilized |
| Shelf life | At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing. |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647 |
| Non-cross-reactive with | No cross reactivity with other proteins. |
| Tested applications |
ELISAFlow CytometryIHCIHC-PWB |
| Dilution range | Western blot, 0.25-0.5 μg/ml, Human ELISA, 0.1-0.5 μg/ml, - |
| Immunogen | E.coli-derived mouse Cd14 recombinant protein (Position: D36-A260). Mouse Cd14 shares 68.2% and 85.7% amino acid (aa) sequence identity with human and rat CD14, respectively. |
| Purification | Immunogen affinity purified. |
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Reactivity & Application Validation
1 species| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Human |
Specifications
Target
E3 ubiquitin-protein ligase Jade-2
Also known as
JADE2; KIAA0239; PHF15; E3 ubiquitin-protein ligase Jade-2; Jade family PHD finger protein 2; PHD finger protein 15
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Human
Tested applications
ELISAFlow CytometryIHCIHC-PWB
Immunogen
E.coli-derived mouse Cd14 recombinant protein (Position: D36-A260). Mouse Cd14 shares 68.2% and 85.7% amino acid (aa) sequence identity with human and rat CD14, respectively.
Observed band
120 kDa
Dilution range
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
ELISA, 0.1-0.5 μg/ml, -
Purification
Immunogen affinity purified.
Storage & Stability
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Long-term storage
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Storage conditions
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Storage buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Shelf life
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Compliance & Certifications
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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