Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-23658

Anti-PD-L1/Cd274 Antibody Picoband®

ABMIUM Verified™|Collaborator Tested|Boster Bio

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Anti-PD-L1/Cd274 Antibody Picoband® Boster Bio
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Anti-PD-L1/Cd274 Antibody Picoband® Boster Bio

Product Description

Anti-PD-L1/Cd274 Antibody Picoband® is a research-use antibody from Boster Bio for studying Programmed cell death 1 ligand 1 in ELISA, Flow Cytometry, IHC, IHC-P, WB workflows with listed reactivity for Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-PD-L1/Cd274 Antibody Picoband® catalog # A00109-3. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Programmed death-ligand 1 (PD-L1), also known as CD274 or B7-H1, is a protein that in humans is encoded by the CD274 gene. It is mapped to 9p24.1. PD-L1 is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. It has been concluded that upregulation of PD-L1 on tumor MDCs downregulates T-cell immunity and that PD-L1 blockade may represent an approach for cancer immunotherapy. Additionally, PD-L1 can provide positive costimulatory signals for innate and adaptive immunity and for protection against intracellular bacterial infection. What’s more, it has been found that PD1/PDL1 pathway may be a good target for restoring antitumor immunity in ovarian cancer.

Product information

Key Facts

Product typePrimary Antibodies Target: Programmed cell death 1 ligand 1
Collaborator Boster Bio
Partner model A00109-3
Target Programmed cell death 1 ligand 1
Reactivity Mouse
Applications ELISA,Flow Cytometry,IHC,IHC-P,WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Cross-reactivityNo cross-reactivity with other proteins.
Dilution rangeWestern blot, 0.25-0.5μg/ml, Mouse<br> ELISA, 0.1-0.5μg/ml, -<br>
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Immunogen E.coli-derived mouse PD-L1/Cd274 recombinant protein (Position: E45-H239).
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Shelf life Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
AvailabilityAvailable
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Mouse

Specifications

Target
Programmed cell death 1 ligand 1
Also known as
B7 H; B7 H1; B7 homolog 1; B7H1; CD274; CD274 molecule; PD L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PD-L1/CD274; Programmed death ligand 1
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Mouse
Tested applications
ELISAFlow CytometryIHCIHC-PWB
Immunogen
E.coli-derived mouse PD-L1/Cd274 recombinant protein (Position: E45-H239).
Observed band
40 kDa
Dilution range
Western blot, 0.25-0.5μg/ml, Mouse
ELISA, 0.1-0.5μg/ml, -
Purification
Immunogen affinity purified.

Storage & Stability

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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