Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-27090

Anti-PCNA Antibody (Monoclonal, PC 10)

ABMIUM Verified™|Collaborator Tested|Boster Bio

Primary Antibody supplied by Boster Bio Read more ›

Anti-PCNA Antibody (Monoclonal, PC 10) Boster Bio
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Anti-PCNA Antibody (Monoclonal, PC 10) Boster Bio

Product Description

Anti-PCNA Antibody (Monoclonal, PC 10) is a research-use antibody from Boster Bio for studying Proliferating cell nuclear antigen in IF/ICC, IHC, IHC-F, IHC-P, WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-PCNA Antibody (Monoclonal, PC 10) catalog # MA1083. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Proliferating cell nuclear antigen (PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA/cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.

Product information

Key Facts

Product typePrimary Antibodies Target: Proliferating cell nuclear antigen
Collaborator Boster Bio
Partner model MA1083
Target Proliferating cell nuclear antigen
Reactivity Human,Mouse,Rat
Applications IF/ICC,IHC,IHC-F,IHC-P,WB
ConjugationUnconjugated
Cross-reactivityNo cross-reactivity with other proteins
Dilution rangeImmunohistochemistry (Paraffin-embedded Section), 0.4-1μg/ml, Human, mouse, rat <br>Immunocytochemistry , 1μg/ml, Human, mouse, rat, -<br>Immunohistochemistry (Frozen Section), 0.4-1μg/ml, Human, mouse, rat, -<br>Western blot, 2μg/ml, Human, mouse, rat<br>
Host Mouse
Clonality Monoclonal
Clone Clone: PC 10
Isotype Mouse IgG2a
Immunogen Protein A fusion protein.
Concentration Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Purification Ascites
Purity No cross reactivity with other proteins.
Shelf life Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
AvailabilityAvailable
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human
Mouse
Rat

Specifications

Target
Proliferating cell nuclear antigen
Also known as
Cyclin; DNA POLYMERASE DELTA AUXILIARY PROTEIN
Concentration
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: PC 10
Isotype
Mouse IgG2a
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
IF/ICCIHCIHC-FIHC-PWB
Immunogen
Protein A fusion protein.
Observed band
35 kDa
Dilution range
Immunohistochemistry (Paraffin-embedded Section), 0.4-1μg/ml, Human, mouse, rat
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Immunohistochemistry (Frozen Section), 0.4-1μg/ml, Human, mouse, rat, -
Western blot, 2μg/ml, Human, mouse, rat
Purification
Ascites

Storage & Stability

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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