Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-27085

Anti-P53 Tp53 Monoclonal Antibody

ABMIUM Verified™|Collaborator Tested|Boster Bio

Primary Antibody supplied by Boster Bio Read more ›

Anti-P53 Tp53 Monoclonal Antibody Boster Bio
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Anti-P53 Tp53 Monoclonal Antibody Boster Bio

Product Description

Anti-P53 Tp53 Monoclonal Antibody is a research-use antibody from Boster Bio for studying Cellular tumor antigen p53 in IF/ICC, IHC, IHC-F, IHC-P, WB workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-P53 Tp53 Monoclonal Antibody catalog # MA1078. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

The p53 tumor antigen is found in increased amounts in a wide variety of transformed cells. The protein is also detectable in many actively proliferating, nontransformed cells, but it is undetectable or present at low levels in resting cells. This protein induces cell cycle arrest or apoptosis in response to sublethal or severe DNA damage, respectively, by differential transcription of target genes and through transcription-independent apoptotic functions. The p53 protein contains 393 amino acids. Human p53 tumour antigen is Locatedto band 17p13. p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis.

Product information

Key Facts

Product typePrimary Antibodies Target: Cellular tumor antigen p53
Collaborator Boster Bio
Partner model MA1078
Target Cellular tumor antigen p53
Reactivity Human
Applications IF/ICC,IHC,IHC-F,IHC-P,WB
ConjugationUnconjugated
Cross-reactivityNo cross-reactivity with other proteins
Dilution rangeImmunohistochemistry (Paraffin-embedded Section), 0.5-2μg/ml, Human <br>Immunocytochemistry , 1μg/ml, Human, -<br>Immunohistochemistry (Frozen Section), 0.5-2μg/ml, Human, -<br>Western blot, 0.5-1μg/ml, Human<br>
Host Mouse
Clonality Monoclonal
Clone Clone: BP53-12
Isotype Mouse IgG2a
Immunogen Recombinant human wild-type p53 protein.
Concentration Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Purification Ascites
Purity No cross reactivity with other proteins.
Shelf life Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
AvailabilityAvailable
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human

Specifications

Target
Cellular tumor antigen p53
Also known as
LFS1; TRP53; Li-Fraumeni syndrome; TRANSFORMATION-RELATED PROTEIN 53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
Concentration
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: BP53-12
Isotype
Mouse IgG2a
Conjugation
Unconjugated
Species reactivity
Human
Tested applications
IF/ICCIHCIHC-FIHC-PWB
Immunogen
Recombinant human wild-type p53 protein.
Observed band
53 kDa
Dilution range
Immunohistochemistry (Paraffin-embedded Section), 0.5-2μg/ml, Human
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry (Frozen Section), 0.5-2μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human
Purification
Ascites

Storage & Stability

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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