Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-22460

Anti-nmt55 p54nrb NONO Antibody Picoband® (monoclonal, 11E2

ABMIUM Verified™|Collaborator Tested|Boster Bio

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Anti-nmt55 p54nrb NONO Antibody Picoband® (monoclonal, 11E2 Boster Bio
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Anti-nmt55 p54nrb NONO Antibody Picoband® (monoclonal, 11E2 Boster Bio

Product Description

Anti-nmt55 p54nrb NONO Antibody Picoband® (monoclonal, 11E2 is a research-use antibody from Boster Bio for studying Non-POU domain-containing octamer-binding protein in ELISA, Flow Cytometry, IF/ICC, IHC, IHC-P, WB workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-nmt55 p54nrb NONO Antibody Picoband® (monoclonal, 11E2) catalog # M03515. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Non-POU domain-containing octamer-binding protein is a protein that in humans is encoded by the NONO gene. This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.

Product information

Key Facts

Product typePrimary Antibodies Target: Non-POU domain-containing octamer-binding protein
Collaborator Boster Bio
Partner model M03515
Target Non-POU domain-containing octamer-binding protein
Reactivity Human
Applications ELISA,Flow Cytometry,IF/ICC,IHC,IHC-P,WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Cross-reactivityNo cross-reactivity with other proteins.
Dilution rangeWestern blot, 0.1-0.5μg/ml<br> Immunocytochemistry, 0.5-1μg/ml<br> Immunocytochemistry/Immunofluorescence, 5μg/ml<br> Flow Cytometry (Fixed), 1-3μg/1x10<sup>6</sup> cells<br>
Host Mouse
Clonality Monoclonal
Clone Clone: 11E2
Isotype Mouse IgG1
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human nmt55 p54nrb, identical to the related mouse and rat sequences.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Purity No cross reactivity with other proteins.
Shelf life Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
AvailabilityAvailable
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human

Specifications

Target
Non-POU domain-containing octamer-binding protein
Also known as
55 kDa nuclear protein; NONO; NonO protein; NRB54; P54; p54(nrb); P54NRB
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: 11E2
Isotype
Mouse IgG1
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Human
Tested applications
ELISAFlow CytometryIF/ICCIHCIHC-PWB
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human nmt55 p54nrb, identical to the related mouse and rat sequences.
Observed band
60 kDa
Dilution range
Western blot, 0.1-0.5μg/ml
Immunocytochemistry, 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Purification
Immunogen affinity purified.

Storage & Stability

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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