Anti-NF45/ILF2 Antibody Picoband®
Primary Antibody supplied by Boster Bio Read more ›

Product Description
Interleukin enhancer-binding factor 2 is a protein that in humans is encoded by the ILF2 gene. The protein encoded by this gene is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The encoded 45 kDa protein (NF45, ILF2) forms a complex with the 90 kDa interleukin enhancer-binding factor 3 (NF90, ILF3), and this complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm, to repair DNA breaks by nonhomologous end joining, and to negatively regulate the microRNA processing pathway. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. Alternative splicing results in multiple transcript variants. Related pseudogenes have been found on chromosomes 3 and 14.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | A04443-2 |
| Product category | Primary Antibodies |
| Reactive species | Human, Mouse, Rat, Monkey |
| Tested applications | ELISA, Flow Cytometry, IF/ICC, IHC, IHC-P, WB |
| Available conjugations | Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647 |
| Gene name | ILF2 |
| Protein name | Interleukin enhancer-binding factor 2 |
| Immunogen | E.coli-derived human Pyruvate Carboxylase/PC recombinant protein (Position: R88-D1165). Human PC shares 97% and 96.6% amino acid (aa) sequence identity with mouse and rat PC, respectively. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Immunogen affinity purified. |
| Storage | At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing. |
| UniProt | Q12905 |
| NCBI Gene ID | 3608 |
Available variants
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μg/vial |
| Biotin | 100 μg/vial |
| Cy3 | 100 μg/vial |
| Fluoro488 | 100 μg/vial |
| Fluoro550 | 100 μg/vial |
| Fluoro594 | 100 μg/vial |
| FITC | 100 μg/vial |
| HRP | 100 μg/vial |
| APC | 100 μg/vial |
| PE | 100 μg/vial |
| Fluoro647 | 100 μg/vial |
Key Facts
| Target | Interleukin enhancer-binding factor 2 |
| Also known as | ILF2; NF45; PRO3063 |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Species reactivity | Human,Mouse,Rat,Monkey |
| Observed band | 43 kDa |
| Form / buffer | Lyophilized |
| Shelf life | At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing. |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647 |
| Non-cross-reactive with | No cross reactivity with other proteins. |
| Tested applications |
ELISAFlow CytometryIF/ICCIHCIHC-PWB |
| Dilution range | Western blot, 0.1-0.25 μg/ml, Human, Monkey, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 1-2 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg /1x106 cells, Human ELISA, 0.1-0.5 μg/ml, - |
| Immunogen | E.coli-derived human Pyruvate Carboxylase/PC recombinant protein (Position: R88-D1165). Human PC shares 97% and 96.6% amino acid (aa) sequence identity with mouse and rat PC, respectively. |
| Purification | Immunogen affinity purified. |
Reactivity & Application Validation
4 speciess| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Human | ||||||||
| Mouse | ||||||||
| Rat | ||||||||
| Monkey |
Specifications
Immunohistochemistry (Paraffin-embedded Section), 1-2 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg /1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Storage & Stability
Compliance & Certifications
Not intended for diagnostic or therapeutic use.