Collaborator Boster Bio
Primary AntibodyABMIUM Verified™Collaborator Tested
ABM-34427

Anti-NEK5 Antibody Picoband®

ABMIUM Verified™|Collaborator Tested|Boster Bio

Primary Antibody supplied by Boster Bio Read more ›

Anti-NEK5 Antibody Picoband® Boster Bio
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Anti-NEK5 Antibody Picoband® Boster Bio

Product Description

Anti-NEK5 Antibody Picoband® is a research-use antibody from Boster Bio for studying Serine/threonine-protein kinase Nek5 in ELISA, Flow Cytometry, IF/ICC, IHC, IHC-P, WB workflows with listed reactivity for Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-NEK5 Antibody Picoband® catalog # A14002-3. Tested in WB, IHC, ICC/IF, ELISA applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Enables protein kinase activity. Acts upstream of or within positive regulation of striated muscle cell differentiation. Predicted to be located in sperm flagellum. Is expressed in choroid invagination; diencephalon roof plate; medulla oblongata part of 4th ventricle choroid plexus; and metencephalon part of 4th ventricle choroid plexus. Orthologous to human NEK5 (NIMA related kinase 5).

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference A14002-3
Product category Primary Antibodies
Reactive species Mouse, Rat
Tested applications ELISA, Flow Cytometry, IF/ICC, IHC, IHC-P, WB
Available conjugations Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647
Gene name NEK5
Protein name Serine/threonine-protein kinase Nek5
Immunogen E.coli-derived mouse NEK5 recombinant protein (Position: K22-Q627). Mouse NEK5 shares 80.8% amino acid (aa) sequence identity with human NEK5.
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Purification Immunogen affinity purified.
Storage At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
UniProt Q7TSC3
NCBI Gene ID 330721

Available variants

Conjugation Size
Unconjugated 100 μg/vial
Biotin 100 μg/vial
Cy3 100 μg/vial
Fluoro488 100 μg/vial
Fluoro550 100 μg/vial
Fluoro594 100 μg/vial
FITC 100 μg/vial
HRP 100 μg/vial
APC 100 μg/vial
PE 100 μg/vial
Fluoro647 100 μg/vial

Key Facts

Product typePrimary Antibody Target: Serine/threonine-protein kinase Nek5
Collaborator Boster Bio
Partner model A14002-3
Target Serine/threonine-protein kinase Nek5
Reactivity Mouse,Rat
Applications ELISA,Flow Cytometry,IF/ICC,IHC,IHC-P,WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Cross-reactivityNo cross-reactivity with other proteins
Dilution rangeWestern blot, 0.25-0.5 μg/ml, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Mouse<br> ELISA, 0.1-0.5 μg/ml<br>
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Immunogen E.coli-derived mouse NEK5 recombinant protein (Position: K22-Q627). Mouse NEK5 shares 80.8% amino acid (aa) sequence identity with human NEK5.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Shelf life At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
AvailabilityAvailable
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Mouse
Rat

Specifications

Target
Serine/threonine-protein kinase Nek5
Also known as
Nek5; Serine/threonine-protein kinase Nek5; EC 2.7.11.1; Never in mitosis A-related kinase 5; NimA-related protein kinase 5
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Mouse,Rat
Tested applications
ELISAFlow CytometryIF/ICCIHCIHC-PWB
Immunogen
E.coli-derived mouse NEK5 recombinant protein (Position: K22-Q627). Mouse NEK5 shares 80.8% amino acid (aa) sequence identity with human NEK5.
Observed band
80 kDa
Dilution range
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Mouse
ELISA, 0.1-0.5 μg/ml
Purification
Immunogen affinity purified.

Storage & Stability

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Storage buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Shelf life
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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