Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-27077

Anti-Nebulin Antibody (Monoclonal, NB2)

ABMIUM Verified™|Collaborator Tested|Boster Bio

Primary Antibody supplied by Boster Bio Read more ›

Anti-Nebulin Antibody (Monoclonal, NB2) Boster Bio
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Anti-Nebulin Antibody (Monoclonal, NB2) Boster Bio

Product Description

Anti-Nebulin Antibody (Monoclonal, NB2) is a research-use antibody from Boster Bio for studying Nebulin in WB workflows with listed reactivity for Chicken. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-Nebulin Antibody (Monoclonal, NB2) catalog # MA1069. Tested in WB applications. This antibody reacts with Chicken.

Nebulin is a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates, nebulin accounts for 3 to 4% of the total myofibrillar protein and its size varies from 600 to 800 kD in a manner that is tissue-, species-, and developmental stage-specific. The nebulin gene contains 183 exons in a 249-kb genomic region. Nebulin is mapped to chromosome 2. Nebulin is a giant filamentous protein specific for vertebrate skeletal muscles. Mutations in the nebulin gene associated with autosomal recessive nemaline myopathy.

Product information

Key Facts

Product typePrimary Antibodies Target: Nebulin
Collaborator Boster Bio
Partner model MA1069
Target Nebulin
Reactivity Chicken
Applications WB
ConjugationUnconjugated
Cross-reactivityNo cross-reactivity with other proteins
Dilution rangeWestern blot, 1-2 μg/ml, Chicken<br>
Host Mouse
Clonality Monoclonal
Clone Clone: NB2
Isotype Mouse IgG1
Immunogen Chicken breast nebulin.
Concentration Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Purification Ascites
Purity No cross reactivity with other proteins.
Shelf life Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
AvailabilityAvailable
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Chicken

Specifications

Target
Nebulin
Also known as
NEB; NEB177D; nebulin; NEM2
Concentration
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: NB2
Isotype
Mouse IgG1
Conjugation
Unconjugated
Species reactivity
Chicken
Tested applications
WB
Immunogen
Chicken breast nebulin.
Observed band
773 kDa
Dilution range
Western blot, 1-2 μg/ml, Chicken
Purification
Ascites

Storage & Stability

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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