ABM-27077
Anti-Nebulin Antibody (Monoclonal, NB2)
ABMIUM Verified™|Collaborator Tested|Boster Bio
Primary Antibody supplied by Boster Bio Read more ›
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Product Description
Boster Bio Anti-Nebulin Antibody (Monoclonal, NB2) catalog # MA1069. Tested in WB applications. This antibody reacts with Chicken.
Nebulin is a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates, nebulin accounts for 3 to 4% of the total myofibrillar protein and its size varies from 600 to 800 kD in a manner that is tissue-, species-, and developmental stage-specific. The nebulin gene contains 183 exons in a 249-kb genomic region. Nebulin is mapped to chromosome 2. Nebulin is a giant filamentous protein specific for vertebrate skeletal muscles. Mutations in the nebulin gene associated with autosomal recessive nemaline myopathy.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | MA1069 |
| Product category | Primary Antibodies, Mouse Monoclonal Antibodies |
| Reactive species | Chicken |
| Tested applications | WB |
| Available conjugations | Unconjugated |
| Gene name | NEB |
| Protein name | Nebulin |
| Specificity | No cross reactivity with other proteins. |
| Immunogen | Chicken breast nebulin. |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Purification | Ascites |
| Storage | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| UniProt | P20929 |
| NCBI Gene ID | Human(4703), Mouse(17996), Rat(311029), Chicken(374027) |
Available variants
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μg/vial |
Key Facts
Product type
Primary Antibody›
Target: Nebulin
| Target | Nebulin |
| Also known as | NEB; NEB177D; nebulin; NEM2 |
| Concentration | Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml. |
| Species reactivity | Chicken |
| Observed band | 773 kDa |
| Purity | No cross reactivity with other proteins. |
| Form / buffer | Lyophilized |
| Shelf life | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| Host species | Mouse |
| Clonality | Monoclonal |
| Clone number | Clone: NB2 |
| Isotype | Mouse IgG1 |
| Conjugation | Unconjugated |
| Non-cross-reactive with | No cross-reactivity with other proteins |
| Tested applications |
WB |
| Dilution range | Western blot, 1-2 μg/ml, Chicken |
| Immunogen | Chicken breast nebulin. |
| Purification | Ascites |
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Reactivity & Application Validation
1 species| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Chicken |
Specifications
Target
Nebulin
Also known as
NEB; NEB177D; nebulin; NEM2
Concentration
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: NB2
Isotype
Mouse IgG1
Conjugation
Unconjugated
Species reactivity
Chicken
Tested applications
WB
Immunogen
Chicken breast nebulin.
Observed band
773 kDa
Dilution range
Western blot, 1-2 μg/ml, Chicken
Purification
Ascites
Storage & Stability
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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