Collaborator Boster Bio
Primary AntibodyABMIUM Verified™Collaborator Tested
ABM-29432

Anti-LSD2 / AOF1 Monoclonal Antibody

ABMIUM Verified™|Collaborator Tested|Boster Bio

Primary Antibody supplied by Boster Bio Read more ›

Anti-LSD2 / AOF1 Monoclonal Antibody Boster Bio
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Anti-LSD2 / AOF1 Monoclonal Antibody Boster Bio

Product Description

Anti-LSD2 / AOF1 Monoclonal Antibody is a research-use antibody from Boster Bio for studying Lysine-specific histone demethylase 2 in Flow Cytometry, IF/ICC, IP, WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-LSD2 / AOF1 Monoclonal Antibody catalog # M08071. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference M08071
Product category Primary Antibodies, Rabbit Monoclonal Antibodies
Reactive species Human, Mouse, Rat
Tested applications Flow Cytometry, IF/ICC, IP, WB
Available conjugations Unconjugated
Gene name KDM1B
Protein name Lysine-specific histone demethylase 2
Immunogen A synthesized peptide derived from human LSD2 / AOF1 Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.
Host Rabbit
Clonality Monoclonal
Isotype Rabbit IgG
Purification Affinity-chromatography
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
UniProt Q8NB78
NCBI Gene ID 221656

Available variants

Conjugation Size
Unconjugated 100 μl/vial

Key Facts

Product typePrimary Antibody Target: Lysine-specific histone demethylase 2
Collaborator Boster Bio
Partner model M08071
Target Lysine-specific histone demethylase 2
Reactivity Human,Mouse,Rat
Applications Flow Cytometry,IF/ICC,IP,WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000<br>ICC/IF 1:50-200<br>IP 1:20<br>FC 1:20<br>
Host Rabbit
Clonality Monoclonal
Clone Clone: ADAG-11
Isotype Rabbit IgG
Immunogen A synthesized peptide derived from human LSD2 / AOF1 Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.
Concentration 0.5mg/ml
Purification Affinity-chromatography
Shelf life Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
AvailabilityAvailable
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human
Mouse
Rat

Specifications

Target
Lysine-specific histone demethylase 2
Also known as
lysine demethylase 1B; AOF1; C6orf193; LSD2; KDM1B
Concentration
0.5mg/ml
Form / buffer
Liquid
Host species
Rabbit
Clonality
Monoclonal
Clone number
Clone: ADAG-11
Isotype
Rabbit IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
Flow CytometryIF/ICCIPWB
Immunogen
A synthesized peptide derived from human LSD2 / AOF1 Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.
Observed band
93 kDa
Dilution range
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
FC 1:20
Purification
Affinity-chromatography

Storage & Stability

Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Storage buffer
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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