Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-23135

Anti-liver Arginase/ARG1 Antibody Picoband® (monoclonal, 2B12

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Anti-liver Arginase/ARG1 Antibody Picoband® (monoclonal, 2B12 Boster Bio
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Anti-liver Arginase/ARG1 Antibody Picoband® (monoclonal, 2B12 Boster Bio

Product Description

Anti-liver Arginase/ARG1 Antibody Picoband® (monoclonal, 2B12 is a research-use antibody from Boster Bio for studying Arginase-1 in ELISA, Flow Cytometry, IHC, IHC-P, WB workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-liver Arginase/ARG1 Antibody Picoband® (monoclonal, 2B12) catalog # M01106-4. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

ARG1 (arginase, live) is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. The isoform encoded byARG1, referred to as the liver, or A-I, isoform, contributes 98% of the arginase activity in liver but is also present in red cells. Using a rat liverARG1cDNA clone to probe a human liver cDNA library,Haraguchi et al. (1987)isolated and characterized a cDNA corresponding to theARG1gene.The ARG1 gene is mapped on 6q23.2 and the arginase gene contains 8 exons. By immunologic studies, 90% of the arginase in red blood cell and liver was precipitated by the antibody, whereas only 50% of the arginase in kidney, brain, and the gastrointestinal tract reacted with it. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.

Product information

Key Facts

Product typePrimary Antibodies Target: Arginase-1
Collaborator Boster Bio
Partner model M01106-4
Target Arginase-1
Reactivity Human,Mouse,Rat,Monkey
Applications ELISA,Flow Cytometry,IHC,IHC-P,WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Cross-reactivityNo cross-reactivity with other proteins.
Dilution rangeWestern blot, 0.1-0.5μg/ml, Monkey, Mouse, Rat<br>Flow Cytometry (Fixed), 1-3μ/1x10<sup>6</sup> cells, Human
Host Mouse
Clonality Monoclonal
Clone Clone: 2B12
Isotype Mouse IgG2b
Immunogen E.coli-derived human liver Arginase/ARG1 recombinant protein (Position: E25-D183).
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Shelf life Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
AvailabilityAvailable
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Application Validation Matrix

4 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human
Mouse
Rat
Monkey

Specifications

Target
Arginase-1
Also known as
ARG1; Arginase 1; Arginase-1; liver Arginase; Liver type arginase
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form / buffer
Lyophilized
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: 2B12
Isotype
Mouse IgG2b
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Human,Mouse,Rat,Monkey
Tested applications
ELISAFlow CytometryIHCIHC-PWB
Immunogen
E.coli-derived human liver Arginase/ARG1 recombinant protein (Position: E25-D183).
Observed band
35 kDa
Dilution range
Western blot, 0.1-0.5μg/ml, Monkey, Mouse, Rat
Flow Cytometry (Fixed), 1-3μ/1x106 cells, Human
Purification
Immunogen affinity purified.

Storage & Stability

Add 0.2ml of distilled water will yield a concentration of 500 μg/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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