Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-22207

Anti-Insulin degrading enzyme/IDE Antibody Picoband®

ABMIUM Verified™|Collaborator Tested|Boster Bio

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Anti-Insulin degrading enzyme/IDE Antibody Picoband® Boster Bio
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Anti-Insulin degrading enzyme/IDE Antibody Picoband® Boster Bio

Product Description

Anti-Insulin degrading enzyme/IDE Antibody Picoband® is a research-use antibody from Boster Bio for studying Insulin-degrading enzyme in ELISA, Flow Cytometry, IF/ICC, IHC, IHC-F, IHC-P, WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-Insulin degrading enzyme/IDE Antibody Picoband® catalog # A01358-2. Tested in ELISA, Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Insulin-degrading enzyme, also known as IDE, is an enzyme. This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Product information

Key Facts

Product typePrimary Antibodies Target: Insulin-degrading enzyme
Collaborator Boster Bio
Partner model A01358-2
Target Insulin-degrading enzyme
Reactivity Human,Mouse,Rat
Applications ELISA,Flow Cytometry,IF/ICC,IHC,IHC-F,IHC-P,WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Cross-reactivityNo cross-reactivity with other proteins.
Dilution rangeWestern blot, 0.1-0.5μg/ml<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml<br> Immunohistochemistry (Frozen Section), 0.5-1μg/ml<br> Immunocytochemistry, 0.5-1μg/ml<br> Flow Cytometry (Fixed), 1-3μg/1x10<sup>6</sup> cells<br> ELISA, 0.1-0.5μg/ml<br>
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Immunogen E. coli-derived human IDE recombinant protein (Position: F485-K756).
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Purity No cross reactivity with other proteins.
Shelf life Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
AvailabilityAvailable
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human
Mouse
Rat

Specifications

Target
Insulin-degrading enzyme
Also known as
Abeta degrading protease; IDE; INSULYSIN
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Human,Mouse,Rat
Tested applications
ELISAFlow CytometryIF/ICCIHCIHC-FIHC-PWB
Immunogen
E. coli-derived human IDE recombinant protein (Position: F485-K756).
Observed band
118 kDa
Dilution range
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 0.1-0.5μg/ml
Purification
Immunogen affinity purified.

Storage & Stability

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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