Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-26131

Anti-IFT74 Antibody Picoband®

ABMIUM Verified™|Collaborator Tested|Boster Bio

Primary Antibody supplied by Boster Bio Read more ›

Anti-IFT74 Antibody Picoband® Boster Bio
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Anti-IFT74 Antibody Picoband® Boster Bio

Product Description

Anti-IFT74 Antibody Picoband® is a research-use antibody from Boster Bio for studying Intraflagellar transport protein 74 homolog in ELISA, Flow Cytometry, IHC, IHC-P, WB workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-IFT74 Antibody Picoband® catalog # A08931-2. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Intraflagellar transport protein 74 homolog (IFT74), also known as coiled-coil domain-containing protein 2 (CCDC2) and capillary morphogenesis gene 1 protein (CMG1), is a protein that in humans is encoded by the IFT74 gene. This gene encodes a core intraflagellar transport (IFT) protein which belongs to a multi-protein complex involved in the transport of ciliary proteins along axonemal microtubules. IFT proteins are found at the base of the cilium as well as inside the cilium, where they assemble into long arrays between the ciliary base and tip. This protein, together with intraflagellar transport protein 81, binds and transports tubulin within cilia and is required for ciliogenesis. Naturally occurring mutations in this gene are associated with amyotrophic lateral sclerosis--frontotemporal dementia and Bardet-Biedl Syndrome.

Product information

Key Facts

Product typePrimary Antibodies Target: Intraflagellar transport protein 74 homolog
Collaborator Boster Bio
Partner model A08931-2
Target Intraflagellar transport protein 74 homolog
Reactivity Human,Mouse,Rat,Monkey
Applications ELISA,Flow Cytometry,IHC,IHC-P,WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Cross-reactivityNo cross reactivity with other proteins.
Dilution rangeWestern blot, 0.25-0.5 μg/ml, Human, Monkey, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat<br> Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen E.coli-derived human PELI3 recombinant protein (Position: M1-R375). Human PELI3 shares 90.1% amino acid (aa) sequence identity with mouse PELI3.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Shelf life At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
AvailabilityAvailable
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Application Validation Matrix

4 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human
Mouse
Rat
Monkey

Specifications

Target
Intraflagellar transport protein 74 homolog
Also known as
IFT74; CCDC2; CMG1; Intraflagellar transport protein 74 homolog; Capillary morphogenesis gene 1 protein; CMG-1; Coiled-coil domain-containing protein 2
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Human,Mouse,Rat,Monkey
Tested applications
ELISAFlow CytometryIHCIHC-PWB
Immunogen
E.coli-derived human PELI3 recombinant protein (Position: M1-R375). Human PELI3 shares 90.1% amino acid (aa) sequence identity with mouse PELI3.
Observed band
69 kDa
Dilution range
Western blot, 0.25-0.5 μg/ml, Human, Monkey, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Purification
Immunogen affinity purified.

Storage & Stability

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Storage buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Shelf life
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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