Anti-IFT74 Antibody Picoband®
Primary Antibody supplied by Boster Bio Read more ›

Product Description
Intraflagellar transport protein 74 homolog (IFT74), also known as coiled-coil domain-containing protein 2 (CCDC2) and capillary morphogenesis gene 1 protein (CMG1), is a protein that in humans is encoded by the IFT74 gene. This gene encodes a core intraflagellar transport (IFT) protein which belongs to a multi-protein complex involved in the transport of ciliary proteins along axonemal microtubules. IFT proteins are found at the base of the cilium as well as inside the cilium, where they assemble into long arrays between the ciliary base and tip. This protein, together with intraflagellar transport protein 81, binds and transports tubulin within cilia and is required for ciliogenesis. Naturally occurring mutations in this gene are associated with amyotrophic lateral sclerosis--frontotemporal dementia and Bardet-Biedl Syndrome.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | A08931-2 |
| Product category | Primary Antibodies |
| Reactive species | Human, Mouse, Rat, Monkey |
| Tested applications | ELISA, Flow Cytometry, IHC, IHC-P, WB |
| Available conjugations | Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647 |
| Gene name | IFT74 |
| Protein name | Intraflagellar transport protein 74 homolog |
| Immunogen | E.coli-derived human PELI3 recombinant protein (Position: M1-R375). Human PELI3 shares 90.1% amino acid (aa) sequence identity with mouse PELI3. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification | Immunogen affinity purified. |
| Storage | At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing. |
| UniProt | Q96LB3 |
| NCBI Gene ID | 80173 |
Available variants
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μg/vial |
| Biotin | 100 μg/vial |
| Cy3 | 100 μg/vial |
| Fluoro488 | 100 μg/vial |
| Fluoro550 | 100 μg/vial |
| Fluoro594 | 100 μg/vial |
| FITC | 100 μg/vial |
| HRP | 100 μg/vial |
| APC | 100 μg/vial |
| PE | 100 μg/vial |
| Fluoro647 | 100 μg/vial |
Key Facts
| Target | Intraflagellar transport protein 74 homolog |
| Also known as | IFT74; CCDC2; CMG1; Intraflagellar transport protein 74 homolog; Capillary morphogenesis gene 1 protein; CMG-1; Coiled-coil domain-containing protein 2 |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Species reactivity | Human,Mouse,Rat,Monkey |
| Observed band | 69 kDa |
| Form / buffer | Lyophilized |
| Shelf life | At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing. |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647 |
| Non-cross-reactive with | No cross reactivity with other proteins. |
| Tested applications |
ELISAFlow CytometryIHCIHC-PWB |
| Dilution range | Western blot, 0.25-0.5 μg/ml, Human, Monkey, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, - |
| Immunogen | E.coli-derived human PELI3 recombinant protein (Position: M1-R375). Human PELI3 shares 90.1% amino acid (aa) sequence identity with mouse PELI3. |
| Purification | Immunogen affinity purified. |
Reactivity & Application Validation
4 speciess| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Human | ||||||||
| Mouse | ||||||||
| Rat | ||||||||
| Monkey |
Specifications
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Storage & Stability
Compliance & Certifications
Not intended for diagnostic or therapeutic use.