ABM-27065
Anti-HSP70 Hspa1b Antibody (Monoclonal, BRM-22)
ABMIUM Verified™|Collaborator Tested|Boster Bio
Primary Antibody supplied by Boster Bio Read more ›
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Product Description
Boster Bio Anti-HSP70 Hspa1b Antibody (Monoclonal, BRM-22) catalog # MA1050. Tested in IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Rabbit, Rat.
Heat-shock proteins, or stress proteins, are expressed in response to heat shock and a variety of other stress stimuli including oxidative free radicals and toxic metal ions. Sargent et al. identified a duplicated HSP70 locus in the class III region of the major histocompatibility complex on 6p21.3. A duplicated locus encoding the major heat shock-induced protein HSP70 is located in the major histocompatibility complex (MHC) class III region 92 kilobases (kb) telomeric to the C2 gene. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | MA1050 |
| Product category | Primary Antibodies, Mouse Monoclonal Antibodies |
| Reactive species | Bovine, Human, Mouse, Rabbit, Rat |
| Tested applications | IHC, IHC-F, IHC-P, WB |
| Available conjugations | Unconjugated |
| Gene name | HSPA1A; HSPA1B |
| Protein name | aldehyde dehydrogenase (NAD(+)) |
| Specificity | No cross reactivity with other proteins. |
| Immunogen | HSP70 isolated from bovine brain. |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Purification | Ascites |
| Storage | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| UniProt | P17879 |
| NCBI Gene ID | Human(3303), Mouse(193740), Rat(24472), Human(3304), Mouse(15511), Rat(294254) |
Available variants
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μg/vial |
Key Facts
Product type
Primary Antibody›
Target: aldehyde dehydrogenase (NAD(+))
HSPA1A; HSPA1BUniProt: P17879Gene ID: Human(3303), Mouse(193740), Rat(24472), Human(3304), Mouse(15511), Rat(294254)
| Target | aldehyde dehydrogenase (NAD(+)) |
| Also known as | HSPA1; Hsp70-1; Hsp70-2; Heat shock 70 kDa protein 1/2 |
| Concentration | Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml. |
| Species reactivity | Bovine,Human,Mouse,Rabbit,Rat |
| Observed band | 70 kDa |
| Purity | No cross reactivity with other proteins. |
| Form / buffer | Lyophilized |
| Shelf life | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| Host species | Mouse |
| Clonality | Monoclonal |
| Clone number | Clone: BRM-22 |
| Isotype | Mouse IgG1 |
| Conjugation | Unconjugated |
| Non-cross-reactive with | No cross-reactivity with other proteins |
| Tested applications |
IHCIHC-FIHC-PWB |
| Dilution range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, bovine, rabbit, rat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, bovine, rabbit, rat, - Western blot, 1μg/ml, Human, bovine, mouse, rabbit, rat |
| Immunogen | HSP70 isolated from bovine brain. |
| Purification | Ascites |
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Reactivity & Application Validation
5 speciess| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Bovine | ||||||||
| Human | ||||||||
| Mouse | ||||||||
| Rabbit | ||||||||
| Rat |
Specifications
Target
aldehyde dehydrogenase (NAD(+))
Also known as
HSPA1; Hsp70-1; Hsp70-2; Heat shock 70 kDa protein 1/2
Concentration
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: BRM-22
Isotype
Mouse IgG1
Conjugation
Unconjugated
Species reactivity
Bovine,Human,Mouse,Rabbit,Rat
Tested applications
IHCIHC-FIHC-PWB
Immunogen
HSP70 isolated from bovine brain.
Observed band
70 kDa
Dilution range
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, bovine, rabbit, rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, bovine, rabbit, rat, -
Western blot, 1μg/ml, Human, bovine, mouse, rabbit, rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, bovine, rabbit, rat, -
Western blot, 1μg/ml, Human, bovine, mouse, rabbit, rat
Purification
Ascites
Storage & Stability
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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