Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-24568

Anti-Histone H1.0/H1F0 Antibody Picoband® (monoclonal, 5I3E6

ABMIUM Verified™|Collaborator Tested|Boster Bio

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Anti-Histone H1.0/H1F0 Antibody Picoband® (monoclonal, 5I3E6 Boster Bio
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Anti-Histone H1.0/H1F0 Antibody Picoband® (monoclonal, 5I3E6 Boster Bio

Product Description

Anti-Histone H1.0/H1F0 Antibody Picoband® (monoclonal, 5I3E6 is a research-use antibody from Boster Bio for studying Histone H1.0 in ELISA, Flow Cytometry, IHC, IHC-P, WB workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-Histone H1.0/H1F0 Antibody Picoband® (monoclonal, 5I3E6) catalog # M08821-2. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

H1 histone family, member 0is a member of thehistonefamily of nuclearproteinswhich are a component ofchromatin. In humans, this protein is encoded by theH1F0gene. It is mapped to 22q13.1. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family.

Product information

Key Facts

Product typePrimary Antibodies Target: Histone H1.0
Collaborator Boster Bio
Partner model M08821-2
Target Histone H1.0
Reactivity Human,Mouse
Applications ELISA,Flow Cytometry,IHC,IHC-P,WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Cross-reactivityNo cross-reactivity with other proteins.
Dilution rangeWestern blot, 0.25-0.5 μg/ml, Human<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse<br> Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Human<br>
Host Mouse
Clonality Monoclonal
Clone Clone: 5I3E6
Isotype Mouse IgG2b
Immunogen E.coli-derived human Histone H1.0/H1F0 recombinant protein (Position: K20-K159).
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Shelf life At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
AvailabilityAvailable
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human
Mouse

Specifications

Target
Histone H1.0
Also known as
H1 histone family; member 0; H10; H1F0; H1FV; histone; Histone H1; Histone H1(0); Histone H1.0
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form / buffer
Lyophilized
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: 5I3E6
Isotype
Mouse IgG2b
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Human,Mouse
Tested applications
ELISAFlow CytometryIHCIHC-PWB
Immunogen
E.coli-derived human Histone H1.0/H1F0 recombinant protein (Position: K20-K159).
Observed band
24 kDa
Dilution range
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Purification
Immunogen affinity purified.

Storage & Stability

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Storage buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Shelf life
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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