Primary Antibody supplied by Boster Bio Read more ›
Anti-hIST1/IST1 Antibody Picoband® is a research-use antibody from Boster Bio for studying IST1 homolog in ELISA, Flow Cytometry, IF/ICC, IHC, IHC-P, WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Boster Bio Anti-hIST1/IST1 Antibody Picoband® catalog # A05507-2. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.IST1 homolog is a protein that in humans is encoded by the KIAA0174 gene. This gene encodes a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT). ESCRT functions in vesicle budding, such as that which occurs during membrane abscission in cytokinesis. There is a pseudogene for this gene on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
| Collaborator | Boster Bio |
| Partner model | A05507-2 |
| Target | IST1 homolog |
| Reactivity | Human,Mouse,Rat |
| Applications | ELISA,Flow Cytometry,IF/ICC,IHC,IHC-P,WB |
| Conjugation | Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647 |
| Cross-reactivity | No cross reactivity with other proteins. |
| Dilution range | Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br> |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | E.coli-derived human hIST1/IST1 recombinant protein (Position: E31-D331). Human IST1 shares 94% and 93.1% amino acid (aa) sequence identity with mouse and rat IST1, respectively. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Purification | Immunogen affinity purified. |
| Shelf life | At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing. |
| Availability | Available |
| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | Flow Cytometry | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Human | ||||||||
| Mouse | ||||||||
| Rat |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
For research applications. Not for diagnostic use.
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