Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-27858

Anti-HIF3/HIF3A Antibody Picoband®

ABMIUM Verified™|Collaborator Tested|Boster Bio

Primary Antibody supplied by Boster Bio Read more ›

Anti-HIF3/HIF3A Antibody Picoband® Boster Bio
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Anti-HIF3/HIF3A Antibody Picoband® Boster Bio

Product Description

Anti-HIF3/HIF3A Antibody Picoband® is a research-use antibody from Boster Bio for studying Hypoxia-inducible factor 3-alpha in ELISA, Flow Cytometry, IHC, IHC-P, WB workflows with listed reactivity for Rat, Human. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-HIF3/HIF3A Antibody catalog # PA2145. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Hypoxia-inducible factor 3 alpha, also called MOP7, is a protein that in humans is encoded by the HIF3A gene. HIF3A is mapped to 19q13.32. This gene involved in adaptive response to hypoxia. HIF3A suppresses hypoxia-inducible expression of HIF1A and EPAS1. It binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. This gene functions as an inhibitor of angiogenesis in the cornea.

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference PA2145
Product category Primary Antibodies
Reactive species Rat, Human
Tested applications ELISA, Flow Cytometry, IHC, IHC-P, WB
Available conjugations Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647
Gene name HIF3A
Protein name Hypoxia-inducible factor 3-alpha
Specificity No cross reactivity with other proteins.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human HIF3, different from the related rat and mouse sequences by five amino acids.
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Purification Immunogen affinity purified.
Storage Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
UniProt Q9Y2N7
NCBI Gene ID 64344

Available variants

Conjugation Size
Unconjugated 100 μg/vial
Biotin 100 μg/vial
Cy3 100 μg/vial
Fluoro488 100 μg/vial
Fluoro550 100 μg/vial
Fluoro594 100 μg/vial
FITC 100 μg/vial
HRP 100 μg/vial
APC 100 μg/vial
PE 100 μg/vial
Fluoro647 100 μg/vial

Key Facts

Product typePrimary Antibodies Target: Hypoxia-inducible factor 3-alpha
Collaborator Boster Bio
Partner model PA2145
Target Hypoxia-inducible factor 3-alpha
Reactivity Rat,Human
Applications ELISA,Flow Cytometry,IHC,IHC-P,WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Cross-reactivityNo cross-reactivity with other proteins
Dilution rangeWestern blot, 0.1-0.5μg/ml, Rat, Human<br>
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human HIF3, different from the related rat and mouse sequences by five amino acids.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Purity No cross reactivity with other proteins.
Shelf life Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
AvailabilityAvailable
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Rat
Human

Specifications

Target
Hypoxia-inducible factor 3-alpha
Also known as
hypoxia inducible factor 3 subunit alpha; HIF-3A; HIF3-alpha-1; IPAS; MOP7; PASD7; bHLHe17; HIF3A
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Rat,Human
Tested applications
ELISAFlow CytometryIHCIHC-PWB
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human HIF3, different from the related rat and mouse sequences by five amino acids.
Observed band
49 kDa
Dilution range
Western blot, 0.1-0.5μg/ml, Rat, Human
Purification
Immunogen affinity purified.

Storage & Stability

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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