ABM-29345
Anti-HEC1 NDC80 Monoclonal Antibody
ABMIUM Verified™|Collaborator Tested|Boster Bio
Primary Antibody supplied by Boster Bio Read more ›
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Product Description
Boster Bio Anti-HEC1 NDC80 Monoclonal Antibody catalog # M01731. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | M01731 |
| Product category | Primary Antibodies, Rabbit Monoclonal Antibodies |
| Reactive species | Human, Mouse, Rat |
| Tested applications | IP, WB |
| Available conjugations | Unconjugated |
| Gene name | NDC80 |
| Protein name | Kinetochore protein NDC80 homolog |
| Immunogen | A synthesized peptide derived from human HEC1 Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Purification | Affinity-chromatography |
| Storage | Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles. |
| UniProt | O14777 |
| NCBI Gene ID | 10403 |
Available variants
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μl/vial |
Key Facts
Product type
Primary Antibody›
Target: Kinetochore protein NDC80 homolog
| Target | Kinetochore protein NDC80 homolog |
| Also known as | HEC; HEC1; HsHec1; hsNDC80; Kinetochore protein Hec1; KNTC2; NDC80; TID3 |
| Concentration | 0.5mg/ml |
| Species reactivity | Human,Mouse,Rat |
| Observed band | 68 kDa |
| Form / buffer | Liquid |
| Shelf life | Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles. |
| Host species | Rabbit |
| Clonality | Monoclonal |
| Clone number | Clone: ACBI-14 |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Tested applications |
IPWB |
| Dilution range | WB 1:500-2000 IP 1:20 |
| Immunogen | A synthesized peptide derived from human HEC1 Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. |
| Purification | Affinity-chromatography |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Human | ||||||||
| Mouse | ||||||||
| Rat |
Specifications
Target
Kinetochore protein NDC80 homolog
Also known as
HEC; HEC1; HsHec1; hsNDC80; Kinetochore protein Hec1; KNTC2; NDC80; TID3
Concentration
0.5mg/ml
Form / buffer
Liquid
Host species
Rabbit
Clonality
Monoclonal
Clone number
Clone: ACBI-14
Isotype
Rabbit IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
IPWB
Immunogen
A synthesized peptide derived from human HEC1 Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
Observed band
68 kDa
Dilution range
WB 1:500-2000
IP 1:20
IP 1:20
Purification
Affinity-chromatography
Storage & Stability
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Storage buffer
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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