Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-21104

Anti-GRP94/HSP90B1 Antibody Picoband®

ABMIUM Verified™|Collaborator Tested|Boster Bio

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Anti-GRP94/HSP90B1 Antibody Picoband® Boster Bio
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Anti-GRP94/HSP90B1 Antibody Picoband® Boster Bio

Product Description

Anti-GRP94/HSP90B1 Antibody Picoband® is a research-use antibody from Boster Bio for studying Endoplasmin in ELISA, Flow Cytometry, IF/ICC, IHC, IHC-P, WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-GRP94/HSP90B1 Antibody Picoband® catalog # PB9637. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Heat shock protein 90kDa beta member 1 (HSP90B1), known as endoplasmin, or GRP94, is a chaperone protein that in humans is encoded by the HSP90B1 gene. It is mapped to chromosome 12q23.3. This gene encodes a member of a family of adenosine triphosphate (ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation.

Product information

Key Facts

Product typePrimary Antibodies Target: Endoplasmin
Collaborator Boster Bio
Partner model PB9637
Target Endoplasmin
Reactivity Human,Mouse,Rat
Applications ELISA,Flow Cytometry,IF/ICC,IHC,IHC-P,WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Cross-reactivityNo cross-reactivity with other proteins
Dilution rangeWestern blot, 0.1-0.25μg/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5μg/ml, Human<br> Flow Cytometry (Fixed), 1-3μg/1x10<sup>6</sup> cells, Human<br>
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Immunogen E.coli-derived human GRP94 recombinant protein (Position: R43-H221). Human GRP94 shares 99.4% and 98.9% amino acid (aa) sequence identity with mouse and rat GRP94, respectively.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Purity No cross reactivity with other proteins.
Shelf life Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
AvailabilityAvailable
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human
Mouse
Rat

Specifications

Target
Endoplasmin
Also known as
Cell and organelle markers; ECGP; endoplasmic reticulum marker; Endoplasmin; GP96; gp96 homolog; GRP 94; GRP94; HSP90B1; TRA1; Tumor rejection antigen 1
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Human,Mouse,Rat
Tested applications
ELISAFlow CytometryIF/ICCIHCIHC-PWB
Immunogen
E.coli-derived human GRP94 recombinant protein (Position: R43-H221). Human GRP94 shares 99.4% and 98.9% amino acid (aa) sequence identity with mouse and rat GRP94, respectively.
Observed band
100 kDa
Dilution range
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Purification
Immunogen affinity purified.

Storage & Stability

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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