Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-24841

Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband® (monoclonal, 6I4E7

ABMIUM Verified™|Collaborator Tested|Boster Bio

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Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband® (monoclonal, 6I4E7 Boster Bio
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Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband® (monoclonal, 6I4E7 Boster Bio

Product Description

Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband® (monoclonal, 6I4E7 is a research-use antibody from Boster Bio for studying Phospholipid hydroperoxide glutathione peroxidase GPX4 in ELISA, Flow Cytometry, IHC, IHC-P, WB workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband® (monoclonal, 6I4E7) catalog # M02059-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Glutathione peroxidase 4, also known as GPX4, is an enzyme that in humans is encoded by the GPX4 gene. This gene encodes a member of the glutathione peroxidase protein family. Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. The encoded protein has been identified as a moonlighting protein based on its ability to serve dual functions as a peroxidase as well as a structural protein in mature spermatozoa. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. Alternative splicing results in multiple transcript variants.

Product information

Key Facts

Product typePrimary Antibodies Target: Phospholipid hydroperoxide glutathione peroxidase GPX4
Collaborator Boster Bio
Partner model M02059-1
Target Phospholipid hydroperoxide glutathione peroxidase GPX4
Reactivity Human
Applications ELISA,Flow Cytometry,IHC,IHC-P,WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Cross-reactivityNo cross-reactivity with other proteins.
Dilution rangeWestern blot, 0.25-0.5 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Human<br>
Host Mouse
Clonality Monoclonal
Clone Clone: 6I4E7
Isotype Mouse IgG2b
Immunogen E.coli-derived human Glutathione Peroxidase 4/GPX4 recombinant protein (Position: A30-F197).
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Shelf life At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
AvailabilityAvailable
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human

Specifications

Target
Phospholipid hydroperoxide glutathione peroxidase GPX4
Also known as
Glutathione peroxidase 4; GPx 4; GPX4; GPx-4; GSHPx 4; GSHPx-4; MCSP; PHGPx; snGPx; snPHGPx
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form / buffer
Lyophilized
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: 6I4E7
Isotype
Mouse IgG2b
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Human
Tested applications
ELISAFlow CytometryIHCIHC-PWB
Immunogen
E.coli-derived human Glutathione Peroxidase 4/GPX4 recombinant protein (Position: A30-F197).
Observed band
19 kDa
Dilution range
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Purification
Immunogen affinity purified.

Storage & Stability

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Storage buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Shelf life
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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