Primary Antibody ABMIUM Verified™Collaborator Tested
ABM-24365

Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband®

ABMIUM Verified™|Collaborator Tested|Boster Bio

Primary Antibody supplied by Boster Bio Read more ›

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Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband® Boster Bio
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Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband® Boster Bio

Product Description

Boster Bio Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband® catalog # A02059-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Glutathione peroxidase 4, also known as GPX4, is an enzyme that in humans is encoded by the GPX4 gene. This gene encodes a member of the glutathione peroxidase protein family. Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. The encoded protein has been identified as a moonlighting protein based on its ability to serve dual functions as a peroxidase as well as a structural protein in mature spermatozoa. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. Alternative splicing results in multiple transcript variants.

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference A02059-1
Product category Primary Antibodies
Reactive species Human, Monkey, Mouse, Rat
Tested applications ELISA, Flow Cytometry, IHC, IHC-P, WB
Available conjugations Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647
Gene name GPX4
Protein name Phospholipid hydroperoxide glutathione peroxidase GPX4
Immunogen E.coli-derived human Glutathione Peroxidase 4/GPX4 recombinant protein (Position: A30-F197).
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Purification Immunogen affinity purified.
Storage At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
UniProt P36969
NCBI Gene ID 2879

Available variants

Conjugation Size
Unconjugated 100 μg/vial
Biotin 100 μg/vial
Cy3 100 μg/vial
Fluoro488 100 μg/vial
Fluoro550 100 μg/vial
Fluoro594 100 μg/vial
FITC 100 μg/vial
HRP 100 μg/vial
APC 100 μg/vial
PE 100 μg/vial
Fluoro647 100 μg/vial

Key Facts

Product type Primary Antibody Target: Phospholipid hydroperoxide glutathione peroxidase GPX4
Target Phospholipid hydroperoxide glutathione peroxidase GPX4
Also known as Glutathione peroxidase 4; GPx 4; GPX4; GPx-4; GSHPx 4; GSHPx-4; MCSP; PHGPx; snGPx; snPHGPx
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Species reactivity Human,Monkey,Mouse,Rat
Observed band 19 kDa
Form / buffer Lyophilized
Shelf life At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Host species Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Conjugation Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Non-cross-reactive with No cross-reactivity with other proteins.
Tested applications
ELISAFlow CytometryIHCIHC-PWB
Dilution range Western blot, 0.25-0.5 μg/ml, Human, Monkey, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Immunogen E.coli-derived human Glutathione Peroxidase 4/GPX4 recombinant protein (Position: A30-F197).
Purification Immunogen affinity purified.
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Reactivity & Application Validation

4 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFLOW CYTOMETRYELISAIP
Human
Monkey
Mouse
Rat

Specifications

Target
Phospholipid hydroperoxide glutathione peroxidase GPX4
Also known as
Glutathione peroxidase 4; GPx 4; GPX4; GPx-4; GSHPx 4; GSHPx-4; MCSP; PHGPx; snGPx; snPHGPx
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Human,Monkey,Mouse,Rat
Tested applications
ELISAFlow CytometryIHCIHC-PWB
Immunogen
E.coli-derived human Glutathione Peroxidase 4/GPX4 recombinant protein (Position: A30-F197).
Observed band
19 kDa
Dilution range
Western blot, 0.25-0.5 μg/ml, Human, Monkey, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Purification
Immunogen affinity purified.

Storage & Stability

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Long-term storage
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Storage conditions
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Storage buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Shelf life
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Compliance & Certifications

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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