Manufactured by:Boster Bio
ABMIUM Verified™RUO

Anti-DDX58 Antibody Picoband®

Primary Antibody supplied by Boster Bio

SKU:
ABM21796-1
Product type
Primary Antibodies
TargetAntiviral innate immune response receptor RIG-I
ApplicationsELISA, Flow Cytometry, IF/ICC, IHC, IHC-P, WB
Species / reactivityHuman, Mouse, Rat
PurityNo cross reactivity with other proteins.

Manufacturer provenance

Manufactured by:
Boster Bio
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Anti-DDX58 Antibody Picoband® is a research-use antibody from Boster Bio for studying Antiviral innate immune response receptor RIG-I in ELISA, Flow Cytometry, IF/ICC, IHC, IHC-P, WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-DDX58 Antibody Picoband® catalog # A00244-2. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

RIG-I (retinoic acid-inducible gene I) is a RIG-I-like receptor dsRNA helicase enzyme that is encoded (in humans) by the DDX58 gene. RIG-I is part of the RIG-I-like receptor family, which also includes MDA5 and LGP2, and functions as a pattern recognition receptor that is a sensor for viruses such as influenza A, Sendai virus, and flavivirus. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response.

Product information

Key facts & specifications
Also known as
DDX58; DEAD box protein 58; RIG 1; RIG I; RIG-I
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Cross-reactivity
No cross-reactivity with other proteins.
Dilution range
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Immunogen
E. coli-derived human DDX58 recombinant protein (Position: H871-K925). Human DDX58 shares 83.3% amino acid (aa) sequence identity with mouse DDX58.
Observed band
116 kDa
Molecular weight
106600 MW
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification
Immunogen affinity purified.
Form / buffer
Lyophilized
Sequence fragment
E. coli-derived human DDX58 recombinant protein (Position: H871-K925). Human DDX58 shares 83.3% amino acid (aa) sequence identity with mouse DDX58.
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
A00244-2
Gene symbol
DDX58
Gene ID
23586
UniProt accession
O95786
Storage, stability & shipping
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Storage warning
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Compliance & documents
CE marked
No
Datasheet
Open datasheet
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Boster Bio and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Boster Bio
£328.00