Primary Antibody ABMIUM Verified™Collaborator Tested
ABM-24349

Anti-DDX3/DDX3X Antibody Picoband®

ABMIUM Verified™|Collaborator Tested|Boster Bio

Primary Antibody supplied by Boster Bio Read more ›

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Anti-DDX3/DDX3X Antibody Picoband® Boster Bio
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Anti-DDX3/DDX3X Antibody Picoband® Boster Bio

Product Description

Boster Bio Anti-DDX3/DDX3X Antibody Picoband® catalog # A00751-6. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

ATP-dependent RNA helicase DDX3X is an enzyme that in humans is encoded by the DDX3X gene. The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome.

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference A00751-6
Product category Primary Antibodies
Reactive species Human, Monkey, Mouse, Rat
Tested applications ELISA, Flow Cytometry, IF/ICC, IHC, IHC-P, WB
Available conjugations Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647
Gene name DDX3X
Protein name ATP-dependent RNA helicase DDX3X
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human DDX3/DDX3X, identical to the related mouse sequences.
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Purification Immunogen affinity purified.
Storage At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
UniProt O00571
NCBI Gene ID 1654

Available variants

Conjugation Size
Unconjugated 100 μg/vial
Biotin 100 μg/vial
Cy3 100 μg/vial
Fluoro488 100 μg/vial
Fluoro550 100 μg/vial
Fluoro594 100 μg/vial
FITC 100 μg/vial
HRP 100 μg/vial
APC 100 μg/vial
PE 100 μg/vial
Fluoro647 100 μg/vial

Key Facts

Product type Primary Antibody Target: ATP-dependent RNA helicase DDX3X
Target ATP-dependent RNA helicase DDX3X
Also known as DBX; DDX14; DDX3; DDX3X; DEAD box; X isoform; Helicase like protein 2; HLP2
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Species reactivity Human,Monkey,Mouse,Rat
Observed band 73 kDa
Form / buffer Lyophilized
Shelf life At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Host species Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Conjugation Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Non-cross-reactive with No cross-reactivity with other proteins.
Tested applications
ELISAFlow CytometryIF/ICCIHCIHC-PWB
Dilution range Western blot, 0.1-0.25 μg/ml, Human, Monkey, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human, Mouse, Rat
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human DDX3/DDX3X, identical to the related mouse sequences.
Purification Immunogen affinity purified.
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Reactivity & Application Validation

4 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFLOW CYTOMETRYELISAIP
Human
Monkey
Mouse
Rat

Specifications

Target
ATP-dependent RNA helicase DDX3X
Also known as
DBX; DDX14; DDX3; DDX3X; DEAD box; X isoform; Helicase like protein 2; HLP2
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Human,Monkey,Mouse,Rat
Tested applications
ELISAFlow CytometryIF/ICCIHCIHC-PWB
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human DDX3/DDX3X, identical to the related mouse sequences.
Observed band
73 kDa
Dilution range
Western blot, 0.1-0.25 μg/ml, Human, Monkey, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human, Mouse, Rat
Purification
Immunogen affinity purified.

Storage & Stability

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Long-term storage
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Storage conditions
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Storage buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Shelf life
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Compliance & Certifications

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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