ABM-27050
Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90)
ABMIUM Verified™|Collaborator Tested|Boster Bio
Primary Antibody supplied by Boster Bio Read more ›
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Product Description
Boster Bio Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90) catalog # MA1026. Tested in IF, IHC, WB applications. This antibody reacts with Human.
Intermediate filaments (IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18 (KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | MA1026 |
| Product category | Primary Antibodies, Mouse Monoclonal Antibodies |
| Reactive species | Human |
| Tested applications | IF/ICC, IHC, IHC-F, IHC-P, WB |
| Available conjugations | Unconjugated |
| Gene name | KRT18 |
| Protein name | Keratin, type I cytoskeletal 18 |
| Specificity | No cross reactivity with other proteins. |
| Immunogen | The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines. |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Purification | Ascites |
| Storage | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| UniProt | Q5BJY9 |
| NCBI Gene ID | Human(3875), Mouse(16668), Rat(294853) |
Available variants
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μg/vial |
Key Facts
Product type
Primary Antibody›
Target: Keratin, type I cytoskeletal 18
| Target | Keratin, type I cytoskeletal 18 |
| Also known as | K18; Keratin-18; CK-18; Cytokeratin-18; CYK18; Cell proliferation-inducing gene 46 protein |
| Concentration | Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml. |
| Species reactivity | Human |
| Observed band | 48 kDa |
| Purity | No cross reactivity with other proteins. |
| Form / buffer | Lyophilized |
| Shelf life | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| Host species | Mouse |
| Clonality | Monoclonal |
| Clone number | Clone: CY-90 |
| Isotype | Mouse IgG1 |
| Conjugation | Unconjugated |
| Non-cross-reactive with | No cross-reactivity with other proteins |
| Tested applications |
IF/ICCIHCIHC-FIHC-PWB |
| Dilution range | Western blot, 0.5-1μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human Immunohistochemistry (Frozen Section), 0.5-2μg/ml, Human, - Immunofluorescence, 5 μg/ml, Human |
| Immunogen | The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines. |
| Purification | Ascites |
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Reactivity & Application Validation
1 species| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Human |
Specifications
Target
Keratin, type I cytoskeletal 18
Also known as
K18; Keratin-18; CK-18; Cytokeratin-18; CYK18; Cell proliferation-inducing gene 46 protein
Concentration
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: CY-90
Isotype
Mouse IgG1
Conjugation
Unconjugated
Species reactivity
Human
Tested applications
IF/ICCIHCIHC-FIHC-PWB
Immunogen
The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.
Observed band
48 kDa
Dilution range
Western blot, 0.5-1μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human
Immunohistochemistry (Frozen Section), 0.5-2μg/ml, Human, -
Immunofluorescence, 5 μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human
Immunohistochemistry (Frozen Section), 0.5-2μg/ml, Human, -
Immunofluorescence, 5 μg/ml, Human
Purification
Ascites
Storage & Stability
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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