Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-27050

Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90)

ABMIUM Verified™|Collaborator Tested|Boster Bio

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Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90) Boster Bio
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Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90) Boster Bio

Product Description

Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90) is a research-use antibody from Boster Bio for studying Keratin, type I cytoskeletal 18 in IF/ICC, IHC, IHC-F, IHC-P, WB workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90) catalog # MA1026. Tested in IF, IHC, WB applications. This antibody reacts with Human.

Intermediate filaments (IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18 (KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis.

Product information

Key Facts

Product typePrimary Antibodies Target: Keratin, type I cytoskeletal 18
Collaborator Boster Bio
Partner model MA1026
Target Keratin, type I cytoskeletal 18
Reactivity Human
Applications IF/ICC,IHC,IHC-F,IHC-P,WB
ConjugationUnconjugated
Cross-reactivityNo cross-reactivity with other proteins
Dilution rangeWestern blot, 0.5-1μg/ml, Human<br> Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human <br>Immunohistochemistry (Frozen Section), 0.5-2μg/ml, Human, -<br> Immunofluorescence, 5 μg/ml, Human<br>
Host Mouse
Clonality Monoclonal
Clone Clone: CY-90
Isotype Mouse IgG1
Immunogen The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.
Concentration Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Purification Ascites
Purity No cross reactivity with other proteins.
Shelf life Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
AvailabilityAvailable
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human

Specifications

Target
Keratin, type I cytoskeletal 18
Also known as
K18; Keratin-18; CK-18; Cytokeratin-18; CYK18; Cell proliferation-inducing gene 46 protein
Concentration
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: CY-90
Isotype
Mouse IgG1
Conjugation
Unconjugated
Species reactivity
Human
Tested applications
IF/ICCIHCIHC-FIHC-PWB
Immunogen
The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.
Observed band
48 kDa
Dilution range
Western blot, 0.5-1μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human
Immunohistochemistry (Frozen Section), 0.5-2μg/ml, Human, -
Immunofluorescence, 5 μg/ml, Human
Purification
Ascites

Storage & Stability

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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