Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-25381

Anti-Cytokeratin 1/Krt1 Antibody Picoband®

ABMIUM Verified™|Collaborator Tested|Boster Bio

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Anti-Cytokeratin 1/Krt1 Antibody Picoband® Boster Bio
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Anti-Cytokeratin 1/Krt1 Antibody Picoband® Boster Bio

Product Description

Anti-Cytokeratin 1/Krt1 Antibody Picoband® is a research-use antibody from Boster Bio for studying Keratin, type II cytoskeletal 1 in ELISA, Flow Cytometry, IF/ICC, IHC, IHC-P, WB workflows with listed reactivity for Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-Cytokeratin 1/Krt1 Antibody Picoband® catalog # A01639-1. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Keratin 1 (KRT1), also known as Cytokeratin 1, or Keratin, type II cytoskeletal 1, is a member of the keratin family. It is specifically expressed in the spinous and granular layers of the epidermis with family member keratin 10. Mutations in this gene have been associated with the variants ofbullous congenital ichthyosiform erythroderma in which the palms and soles of the feet are affected. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. And this type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Product information

Key Facts

Product typePrimary Antibodies Target: Keratin, type II cytoskeletal 1
Collaborator Boster Bio
Partner model A01639-1
Target Keratin, type II cytoskeletal 1
Reactivity Mouse
Applications ELISA,Flow Cytometry,IF/ICC,IHC,IHC-P,WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Cross-reactivityNo cross-reactivity with other proteins.
Dilution rangeWestern blot, 0.25-0.5 μg/ml, Mouse<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse<br> Immunofluorescence, 5 μg/ml, Mouse<br> ELISA, 0.1-0.5 μg/ml, -<br>
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Immunogen E.coli-derived mouse Cytokeratin 1/Krt1 recombinant protein (Position: L223-Q439).
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Shelf life At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
AvailabilityAvailable
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Mouse

Specifications

Target
Keratin, type II cytoskeletal 1
Also known as
67 kDa cytokeratin; CK 1; CK1; Cytokeratin 1; keratin 1; KRT1
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Mouse
Tested applications
ELISAFlow CytometryIF/ICCIHCIHC-PWB
Immunogen
E.coli-derived mouse Cytokeratin 1/Krt1 recombinant protein (Position: L223-Q439).
Observed band
66 kDa
Dilution range
Western blot, 0.25-0.5 μg/ml, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse
Immunofluorescence, 5 μg/ml, Mouse
ELISA, 0.1-0.5 μg/ml, -
Purification
Immunogen affinity purified.

Storage & Stability

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Storage buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Shelf life
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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