Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-25407

Anti-CRKL Antibody Picoband®

ABMIUM Verified™|Collaborator Tested|Boster Bio

Primary Antibody supplied by Boster Bio Read more ›

Anti-CRKL Antibody Picoband® Boster Bio
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Anti-CRKL Antibody Picoband® Boster Bio

Product Description

Anti-CRKL Antibody Picoband® is a research-use antibody from Boster Bio for studying Crk-like protein in ELISA, Flow Cytometry, IF/ICC, IHC, IHC-P, IP, WB workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-CRKL Antibody Picoband® catalog # A02100-1. Tested in ELISA, Flow Cytometry, IP, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Crk-like protein is a protein that in humans is encoded by the CRKL gene. This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.

Product information

Key Facts

Product typePrimary Antibodies Target: Crk-like protein
Collaborator Boster Bio
Partner model A02100-1
Target Crk-like protein
Reactivity Human
Applications ELISA,Flow Cytometry,IF/ICC,IHC,IHC-P,IP,WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Cross-reactivityNo cross-reactivity with other proteins
Dilution rangeWestern blot, 0.25-0.5 μg/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> Immunoprecipitation, 0.5-2 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Immunogen E.coli-derived human CRKL recombinant protein (Position: M1-H290).
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Shelf life At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
AvailabilityAvailable
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human

Specifications

Target
Crk-like protein
Also known as
CRK like proto-oncogene; adaptor protein; CRKL
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Human
Tested applications
ELISAFlow CytometryIF/ICCIHCIHC-PIPWB
Immunogen
E.coli-derived human CRKL recombinant protein (Position: M1-H290).
Observed band
34 kDa
Dilution range
Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Purification
Immunogen affinity purified.

Storage & Stability

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Storage buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Shelf life
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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