Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-22823

Anti-CD3 epsilon/Cd3e Antibody Picoband®

ABMIUM Verified™|Collaborator Tested|Boster Bio

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Anti-CD3 epsilon/Cd3e Antibody Picoband® Boster Bio
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Anti-CD3 epsilon/Cd3e Antibody Picoband® Boster Bio

Product Description

Anti-CD3 epsilon/Cd3e Antibody Picoband® is a research-use antibody from Boster Bio for studying T-cell surface glycoprotein CD3 epsilon chain in ELISA, Flow Cytometry, IHC, IHC-P, WB workflows with listed reactivity for Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-CD3 epsilon/Cd3e Antibody Picoband® catalog # A02765-1. Tested in ELISA, Flow Cytometry , IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.

Product information

Key Facts

Product typePrimary Antibodies Target: T-cell surface glycoprotein CD3 epsilon chain
Collaborator Boster Bio
Partner model A02765-1
Target T-cell surface glycoprotein CD3 epsilon chain
Reactivity Mouse,Rat
Applications ELISA,Flow Cytometry,IHC,IHC-P,WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Cross-reactivityNo cross-reactivity with other proteins.
Dilution rangeWestern blot, 0.1-0.25μg/ml, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat<br> Flow Cytometry (Fixed), 1-3μg/1x10<sup>6</sup> cells, Mouse<br> ELISA, 0.1-0.5μg/ml, -, Rat<br>
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Immunogen E.coli-derived mouse CD3 epsilon/Cd3e recombinant protein (Position: D22-A113).
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Purity No cross reactivity with other proteins.
Shelf life Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
AvailabilityAvailable
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Mouse
Rat

Specifications

Target
T-cell surface glycoprotein CD3 epsilon chain
Also known as
CD3; CD3 epsilon; CD3E; CD3-epsilon; T3E; TCRE
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Mouse,Rat
Tested applications
ELISAFlow CytometryIHCIHC-PWB
Immunogen
E.coli-derived mouse CD3 epsilon/Cd3e recombinant protein (Position: D22-A113).
Observed band
23 kDa
Dilution range
Western blot, 0.1-0.25μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Mouse
ELISA, 0.1-0.5μg/ml, -, Rat
Purification
Immunogen affinity purified.

Storage & Stability

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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