Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-21378

Anti-CD153/TNFSF8 Antibody

ABMIUM Verified™|Collaborator Tested|Boster Bio

Primary Antibody supplied by Boster Bio Read more ›

Anti-CD153/TNFSF8 Antibody Boster Bio
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Anti-CD153/TNFSF8 Antibody Boster Bio

Product Description

Anti-CD153/TNFSF8 Antibody is a research-use antibody from Boster Bio for studying Tumor necrosis factor ligand superfamily member 8 in ELISA, Flow Cytometry, IHC, IHC-P, WB workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-CD153/TNFSF8 Antibody catalog # RP1103. Tested in Flow Cytometry, IHC applications. This antibody reacts with Human.

CD153 is a ligand for CD30. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Two transcript variants encoding different isoforms have been found for this gene.

Product information

Key Facts

Product typePrimary Antibodies Target: Tumor necrosis factor ligand superfamily member 8
Collaborator Boster Bio
Partner model RP1103
Target Tumor necrosis factor ligand superfamily member 8
Reactivity Human
Applications ELISA,Flow Cytometry,IHC,IHC-P,WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Cross-reactivityNo cross-reactivity with other proteins.
Dilution rangeImmunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml<br>Flow Cytometry, 1-3μg/1x10<sup>6</sup>cells<br>
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Immunogen E.coli-derived human CD153 recombinant protein (Position: Q63-D234). Human CD153 shares 75% amino acid (aa) sequence identity with mouse CD153.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Purity No cross reactivity with other proteins.
Shelf life Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
AvailabilityAvailable
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human

Specifications

Target
Tumor necrosis factor ligand superfamily member 8
Also known as
TNF superfamily member 8; CD153; CD30L; CD30LG; TNLG3A; TNFSF8
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Human
Tested applications
ELISAFlow CytometryIHCIHC-PWB
Immunogen
E.coli-derived human CD153 recombinant protein (Position: Q63-D234). Human CD153 shares 75% amino acid (aa) sequence identity with mouse CD153.
Observed band
86 kDa
Dilution range
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Flow Cytometry, 1-3μg/1x106cells
Purification
Immunogen affinity purified.

Storage & Stability

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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