ABM-27712
Anti-Caspase-3(P17)/CASP3 Antibody Picoband®
ABMIUM Verified™|Collaborator Tested|Boster Bio
Primary Antibody supplied by Boster Bio Read more ›
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Product Description
Boster Bio Anti-Caspase-3 (P17)/CASP3 Antibody catalog # PA1961-1. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
CASP3 (Caspase3 Apoptosis-Related Cysteine Protease), also known as YAMA, CPP32 or APOPAIN, is a caspase protein that interacts with caspase 8 and caspase 9. It is encoded by the CASP3 gene. The CASP3 protein is a member of the cysteine-aspartic acid protease (caspase) family. Tiso et al. (1996) used radiation hybrid mapping to localize the CPP32 gene to human chromosome 4q33-q35.1. Fernandes-Alnemri et al. (1994) found that overexpression of CPP32 in insect cells induced apoptosis. Coexpression of the 2 CPP32 subunits in insect cells also resulted in apoptosis. Tewari et al. (1995) showed that purified human ICE cleaved the Yama proenzyme into a proteolytically active form and that activated Yama cleaved PARP into the 85-kD apoptotic form.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | PA1961-1 |
| Product category | Primary Antibodies |
| Reactive species | Mouse, Rat |
| Tested applications | ELISA, Flow Cytometry, IHC, IHC-P, WB |
| Available conjugations | Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647 |
| Gene name | CASP3 |
| Protein name | Caspase-3 |
| Specificity | No cross reactivity with other proteins. |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of mouse Caspase-3(P17), different from the related rat sequence by one amino acid. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Purification | Immunogen affinity purified. |
| Storage | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| UniProt | P70677 |
| NCBI Gene ID | 12367 |
Available variants
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μg/vial |
| Biotin | 100 μg/vial |
| Cy3 | 100 μg/vial |
| Fluoro488 | 100 μg/vial |
| Fluoro550 | 100 μg/vial |
| Fluoro594 | 100 μg/vial |
| FITC | 100 μg/vial |
| HRP | 100 μg/vial |
| APC | 100 μg/vial |
| PE | 100 μg/vial |
| Fluoro647 | 100 μg/vial |
Key Facts
Product type
Primary Antibody›
Target: Caspase-3
| Target | Caspase-3 |
| Also known as | A830040C14Rik antibody; Apopain antibody; Apopain precursor antibody; CASP 3 antibody; CASP-3 antibody; CASP3 antibody; Casp3a antibody; Caspase 3 antibody; Caspase 3 apoptosis related cysteine protease antibody; Caspase 3 p17 subunit antibody; Caspase 3, apoptosis-related cysteine peptidase antibody; Caspase 3, apoptosis-related cysteine protease antibody; Caspase 3, apoptosis-related cysteine protease a antibody; Caspase-3 subunit p17 antibody; Caspase3 antibody; CC3 antibody; CPP 32 antibody; CPP-32 antibody; CPP32 antibody; CPP32B antibody; Cysteine protease CPP32 antibody; EC 3.4.22.56 antibody; Human cysteine protease CPP32 isoform alpha mRNA complete cds antibody; ICE3 antibody; LICE antibody; mldy antibody; OTTHUMP00000165052 antibody; OTTHUMP00000165053 antibody; OTTHUMP00000165054 antibody; PARP cleavage protease antibody; Procaspase3 antibody; Protein Yama antibody; SCA 1 antibody; SCA-1 antibody; SCA1 antibody; SREBP cleavage activity 1 antibody; Yama antibody; Yama protein antibody |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Species reactivity | Mouse,Rat |
| Observed band | 32 kDa, 19 kDa |
| Purity | No cross reactivity with other proteins. |
| Form / buffer | Lyophilized |
| Shelf life | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647 |
| Non-cross-reactive with | No cross-reactivity with other proteins |
| Tested applications |
ELISAFlow CytometryIHCIHC-PWB |
| Dilution range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse Western blot, 0.1-0.5μg/ml, Mouse, Rat |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of mouse Caspase-3(P17), different from the related rat sequence by one amino acid. |
| Purification | Immunogen affinity purified. |
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Reactivity & Application Validation
2 speciess| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Mouse | ||||||||
| Rat |
Specifications
Target
Caspase-3
Also known as
A830040C14Rik antibody; Apopain antibody; Apopain precursor antibody; CASP 3 antibody; CASP-3 antibody; CASP3 antibody; Casp3a antibody; Caspase 3 antibody; Caspase 3 apoptosis related cysteine protease antibody; Caspase 3 p17 subunit antibody; Caspase 3, apoptosis-related cysteine peptidase antibody; Caspase 3, apoptosis-related cysteine protease antibody; Caspase 3, apoptosis-related cysteine protease a antibody; Caspase-3 subunit p17 antibody; Caspase3 antibody; CC3 antibody; CPP 32 antibody; CPP-32 antibody; CPP32 antibody; CPP32B antibody; Cysteine protease CPP32 antibody; EC 3.4.22.56 antibody; Human cysteine protease CPP32 isoform alpha mRNA complete cds antibody; ICE3 antibody; LICE antibody; mldy antibody; OTTHUMP00000165052 antibody; OTTHUMP00000165053 antibody; OTTHUMP00000165054 antibody; PARP cleavage protease antibody; Procaspase3 antibody; Protein Yama antibody; SCA 1 antibody; SCA-1 antibody; SCA1 antibody; SREBP cleavage activity 1 antibody; Yama antibody; Yama protein antibody
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Mouse,Rat
Tested applications
ELISAFlow CytometryIHCIHC-PWB
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse Caspase-3(P17), different from the related rat sequence by one amino acid.
Observed band
32 kDa, 19 kDa
Dilution range
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Purification
Immunogen affinity purified.
Storage & Stability
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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