Anti-B Raf/BRAF Antibody Picoband®
Primary Antibody supplied by Boster Bio Read more ›

Product Description
BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B-Raf, also known as ONCOGENE BRAF, BRAF1, RAFB1, Proto-oncogene B-Raf p94, v-Raf murine sarcoma viral oncogene homolog B1, proto-oncogene B-Raf, v-Raf murine sarcoma viral oncogene homolog B1, while the protein is more formally known as serine/threonine-protein kinase B-Raf. BRAF deduced 651-amino acid protein has a calculated molecular mass of 72.5 kD. Northern blot analysis detected transcripts of 10 and 13 kb in cerebrum, fetal brain, and placenta and transcripts of 2.6 and 4.5 kb in testis. Testis also showed lower expression of the 10- and 13-kb transcripts.B-Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | PA1848 |
| Product category | Primary Antibodies |
| Reactive species | Human, Mouse, Rat |
| Tested applications | ELISA, Flow Cytometry, IHC, IHC-P, WB |
| Available conjugations | Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647 |
| Gene name | BRAF |
| Protein name | Serine/threonine-protein kinase B-raf |
| Specificity | No cross reactivity with other proteins. |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human B Raf, identical to the related rat and mouse sequences. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Purification | Immunogen affinity purified. |
| Storage | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| UniProt | P15056 |
| NCBI Gene ID | 673 |
Available variants
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μg/vial |
| Biotin | 100 μg/vial |
| Cy3 | 100 μg/vial |
| Fluoro488 | 100 μg/vial |
| Fluoro550 | 100 μg/vial |
| Fluoro594 | 100 μg/vial |
| FITC | 100 μg/vial |
| HRP | 100 μg/vial |
| APC | 100 μg/vial |
| PE | 100 μg/vial |
| Fluoro647 | 100 μg/vial |
Key Facts
| Target | Serine/threonine-protein kinase B-raf |
| Also known as | B RAF1; BRAF; BRAF1; p94; Proto oncogene B Raf; RAFB1 |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Species reactivity | Human,Mouse,Rat |
| Observed band | 84 kDa |
| Purity | No cross reactivity with other proteins. |
| Form / buffer | Lyophilized |
| Shelf life | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647 |
| Non-cross-reactive with | No cross-reactivity with other proteins |
| Tested applications |
ELISAFlow CytometryIHCIHC-PWB |
| Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human B Raf, identical to the related rat and mouse sequences. |
| Purification | Immunogen affinity purified. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Human | ||||||||
| Mouse | ||||||||
| Rat |
Specifications
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
Storage & Stability
Compliance & Certifications
Not intended for diagnostic or therapeutic use.