Anti-Aurora A/AURKA Antibody Picoband®

Boster Bio Anti-Aurora A/AURKA Antibody Picoband® catalog # A00246-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

AURKA(aurora kinase A), also called ARK1, AurA, AIK , AURORA2 ,BTAK, PPP1R47, STK7, STK15,STK6, is a mitotic centrosomal protein kinase. The main role of AURKA in tumor development is in controlling chromosome segregation during mitosis. Aurora A is a member of a family of mitotic serine/threonine kinases. Cell cycle and Northern blot analyses showed that peak expression of AURKA occurs during the G2/M phase and then decreases. By fluorescence in situ hybridization, AURKA gene is represented by 2 signals in chromosome bands 20q13.2-q13.3 and 1q41-q42. The AURKA gene is overexpressed in many human cancers. Ectopic overexpression of Aurora kinase A in mammalian cells induces centrosome amplification, chromosome instability, and oncogenic transformation, a phenotype characteristic of loss-of-function mutations of p53. Depletion of Ajuba prevented activation of AURKA at centrosomes in late G2 phase and inhibited mitotic entry. Activation of AURKA was independently sufficient to induce rapid ciliary resorption, and AURKA acted in this process through phosphorylation of HDAC6, leading to HDAC6-dependent tubulin deacetylation and destabilization of the ciliary axoneme. Small molecule inhibitors of AURKA and HDAC6 reduced regulated disassembly of cilia.

Product information

Supplier	Boster Bio
ABMIUM evidence status	ABMIUM Verified™ collaborator-tested data
Catalogue reference	A00246-3
Product category	Primary Antibodies
Reactive species	Human
Tested applications	ELISA, Flow Cytometry, IF/ICC, IHC, IHC-P, WB
Available conjugations	Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647
Gene name	AURKA
Protein name	Aurora kinase A
Immunogen	E.coli-derived human Aurora A/AURKA recombinant protein (Position: M1-K97).
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Purification	Immunogen affinity purified.
Storage	At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
UniProt	O14965
NCBI Gene ID	6790

Available variants

Conjugation	Size
Unconjugated	100 μg/vial
Biotin	100 μg/vial
Cy3	100 μg/vial
Fluoro488	100 μg/vial
Fluoro550	100 μg/vial
Fluoro594	100 μg/vial
FITC	100 μg/vial
HRP	100 μg/vial
APC	100 μg/vial
PE	100 μg/vial
Fluoro647	100 μg/vial