ABM-29397
Anti-Atg4A Monoclonal Antibody
ABMIUM Verified™|Collaborator Tested|Boster Bio
Primary Antibody supplied by Boster Bio Read more ›
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Product Description
Boster Bio Anti-Atg4A Monoclonal Antibody catalog # M06539. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | M06539 |
| Product category | Primary Antibodies, Rabbit Monoclonal Antibodies |
| Reactive species | Human, Mouse, Rat |
| Tested applications | IHC, IP, WB |
| Available conjugations | Unconjugated |
| Gene name | ATG4A |
| Protein name | Cysteine protease ATG4A |
| Immunogen | A synthesized peptide derived from human Atg4A The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homologue. |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Purification | Affinity-chromatography |
| Storage | Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles. |
| UniProt | Q8WYN0 |
| NCBI Gene ID | 115201 |
Available variants
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μl/vial |
Key Facts
Product type
Primary Antibody›
Target: Cysteine protease ATG4A
| Target | Cysteine protease ATG4A |
| Also known as | APG4A; ATG4A; AUTL2; Autophagin 2; Cysteine protease ATG4A; hAPG4A |
| Concentration | 0.5mg/ml |
| Species reactivity | Human,Mouse,Rat |
| Observed band | 45 kDa |
| Form / buffer | Liquid |
| Shelf life | Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles. |
| Host species | Rabbit |
| Clonality | Monoclonal |
| Clone number | Clone: ACHB-1 |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Tested applications |
IHCIPWB |
| Dilution range | WB 1:500-2000 IHC 1:50-200 IP 1:20 |
| Immunogen | A synthesized peptide derived from human Atg4A The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homologue. |
| Purification | Affinity-chromatography |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Human | ||||||||
| Mouse | ||||||||
| Rat |
Specifications
Target
Cysteine protease ATG4A
Also known as
APG4A; ATG4A; AUTL2; Autophagin 2; Cysteine protease ATG4A; hAPG4A
Concentration
0.5mg/ml
Form / buffer
Liquid
Host species
Rabbit
Clonality
Monoclonal
Clone number
Clone: ACHB-1
Isotype
Rabbit IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
IHCIPWB
Immunogen
A synthesized peptide derived from human Atg4A The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homologue.
Observed band
45 kDa
Dilution range
WB 1:500-2000
IHC 1:50-200
IP 1:20
IHC 1:50-200
IP 1:20
Purification
Affinity-chromatography
Storage & Stability
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Storage buffer
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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