Primary Antibody supplied by Boster Bio Read more ›
Anti-Atg4A Monoclonal Antibody is a research-use antibody from Boster Bio for studying Cysteine protease ATG4A in IHC, IP, WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Boster Bio Anti-Atg4A Monoclonal Antibody catalog # M06539. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | M06539 |
| Product category | Primary Antibodies, Rabbit Monoclonal Antibodies |
| Reactive species | Human, Mouse, Rat |
| Tested applications | IHC, IP, WB |
| Available conjugations | Unconjugated |
| Gene name | ATG4A |
| Protein name | Cysteine protease ATG4A |
| Immunogen | A synthesized peptide derived from human Atg4A The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homologue. |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Purification | Affinity-chromatography |
| Storage | Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles. |
| UniProt | Q8WYN0 |
| NCBI Gene ID | 115201 |
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μl/vial |
| Collaborator | Boster Bio |
| Partner model | M06539 |
| Target | Cysteine protease ATG4A |
| Reactivity | Human,Mouse,Rat |
| Applications | IHC,IP,WB |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000<br>IHC 1:50-200<br>IP 1:20<br> |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | Clone: ACHB-1 |
| Isotype | Rabbit IgG |
| Immunogen | A synthesized peptide derived from human Atg4A The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homologue. |
| Concentration | 0.5mg/ml |
| Purification | Affinity-chromatography |
| Shelf life | Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles. |
| Availability | Available |
| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | Flow Cytometry | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Human | ||||||||
| Mouse | ||||||||
| Rat |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
For research applications. Not for diagnostic use.
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