Anti-Aqp2 Antibody Picoband®

Boster Bio Anti-Aqp2 Antibody Picoband® catalog # A00935. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

AQP2 (Aquaporin 2), also called AQUAPORIN-CD, is found in the apical cell membranes of the kidney's collecting duct principal cells and in intracellular vesicles located throughout the cell. The AQP2 gene is mapped to chromosome 12q13, very close to the site of major intrinsic protein by situ hybridization. The investigators suggested that a defect in the AQP2 gene is the basis of the autosomal form of nephrogenic diabetes insipidus. The functional expression and the limited localization suggested that AQP2 is the vasopressin-regulated water channel. Using rat kidney slices and porcine kidney cells stably expressing rat Aqp2, AQP2 trafficking can be stimulated by cAMP-independent pathways that utilize nitric oxide (NO). The NO donors sodium nitroprusside (SNP) and NONOate and the NO synthase substrate L-arginine mimicked the effect of vasopressin (VP), stimulating relocation of Aqp2 from cytoplasmic vesicles to the apical plasma membrane. SNP increased intracellular cGMP rather than cAMP, and exogenous cGMP stimulated AQP2 membrane insertion. Atrial natriuretic factor, which signals via cGMP, also stimulated AQP2 translocation. AQP2 expression in kidney connecting tubules is sufficient for survival and that AQP2 expression in collecting ducts is required to regulate body water balance. The S256L substitution in the cytoplasmic tail of the Aqp2 protein prevented phosphorylation at S256 and the subsequent accumulation of Aqp2 on the apical membrane of the collecting duct principal cells.

Product information

Supplier	Boster Bio
ABMIUM evidence status	ABMIUM Verified™ collaborator-tested data
Catalogue reference	A00935
Product category	Primary Antibodies
Reactive species	Mouse, Rat
Tested applications	ELISA, Flow Cytometry, IHC, IHC-P, WB
Available conjugations	Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647
Gene name	Aqp2
Protein name	Aquaporin-2
Immunogen	E.coli-derived mouse Aqp2 recombinant protein (Position: A102-A271). Mouse Aqp2 shares 87.6% and 98.2% amino acid (aa) sequence identity with human and rat Aqp2, respectively.
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Purification	Immunogen affinity purified.
Storage	At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
UniProt	P56402
NCBI Gene ID	11827

Available variants

Conjugation	Size
Unconjugated	100 μg/vial
Biotin	100 μg/vial
Cy3	100 μg/vial
Fluoro488	100 μg/vial
Fluoro550	100 μg/vial
Fluoro594	100 μg/vial
FITC	100 μg/vial
HRP	100 μg/vial
APC	100 μg/vial
PE	100 μg/vial
Fluoro647	100 μg/vial