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Anti-Apoptosis repressor with CARD/NOL3 Antibody Picoband® is a research-use antibody from Boster Bio for studying Nucleolar protein 3 in ELISA, Flow Cytometry, IHC, IHC-P, WB workflows with listed reactivity for Human, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Boster Bio Anti-Apoptosis repressor with CARD/NOL3 Antibody catalog # PA1793. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.NOL3 (Nucleolar protein 3), also known as ARC, NOP30, CARD2 and MYP, is a protein that in humans is encoded by the NOL3 gene. NOL3 has been shown to interact with SFRS9 and Caspase 8. By genomic sequence analysis, Stoss et al. (1999) determined that the NOL3 gene, which encodes NOP30 and MYP and which they called NOP, is composed of 4 exons. The alternative 5-prime splice site that generates the 2 isoforms is located in exon 2. It is reported that expression of the ARC cDNA encoding the smaller transcript inhibited apoptosis in a dose-dependent manner when coexpressed with CASP8 but not when coexpressed with CASP9. ARC also inhibited apoptosis induced by stimulation of CD95/FAS, tumor necrosis factor receptor-1, and TRAMP/death receptor-3. Enzymatic analysis showed that ARC inhibits the enzymatic activity of CASP8. Immunoprecipitation and immunoblot analysis indicated that ARC interacts with CASP2 and CASP8 through its N-terminal death effector domain but does not interact with CASP1, CASP3, or CASP9.
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | PA1793 |
| Product category | Primary Antibodies |
| Reactive species | Human, Rat |
| Tested applications | ELISA, Flow Cytometry, IHC, IHC-P, WB |
| Available conjugations | Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647 |
| Gene name | NOL3 |
| Protein name | Nucleolar protein 3 |
| Specificity | No cross reactivity with other proteins. |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Apoptosis repressor with CARD, identical to the related rat sequence, and different from the related mouse sequence by one amino acid. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Purification | Immunogen affinity purified. |
| Storage | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| UniProt | O60936 |
| NCBI Gene ID | 8996 |
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μg/vial |
| Biotin | 100 μg/vial |
| Cy3 | 100 μg/vial |
| Fluoro488 | 100 μg/vial |
| Fluoro550 | 100 μg/vial |
| Fluoro594 | 100 μg/vial |
| FITC | 100 μg/vial |
| HRP | 100 μg/vial |
| APC | 100 μg/vial |
| PE | 100 μg/vial |
| Fluoro647 | 100 μg/vial |
| Collaborator | Boster Bio |
| Partner model | PA1793 |
| Target | Nucleolar protein 3 |
| Reactivity | Human,Rat |
| Applications | ELISA,Flow Cytometry,IHC,IHC-P,WB |
| Conjugation | Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647 |
| Cross-reactivity | No cross-reactivity with other proteins |
| Dilution range | Western blot, 0.1-0.5μg/ml, Human, Rat<br> |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Apoptosis repressor with CARD, identical to the related rat sequence, and different from the related mouse sequence by one amino acid. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Purification | Immunogen affinity purified. |
| Purity | No cross reactivity with other proteins. |
| Shelf life | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| Availability | Available |
| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | Flow Cytometry | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Human | ||||||||
| Rat |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
For research applications. Not for diagnostic use.
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