ABM-27099
Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2)
ABMIUM Verified™|Collaborator Tested|Boster Bio
Primary Antibody supplied by Boster Bio Read more ›
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Product Description
Boster Bio Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2) catalog # MA1105. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1 (HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al. (2001) identified 3 HIP1-associated proteins, clathrin heavy chain (CLTC)and alpha-adaptin A and C. Coat proteins of approximately 100-kD (adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | MA1105 |
| Product category | Primary Antibodies, Mouse Monoclonal Antibodies |
| Reactive species | Human, Mouse, Rat |
| Tested applications | WB |
| Available conjugations | Unconjugated |
| Gene name | AP2A1 |
| Protein name | AP-2 complex subunit alpha |
| Specificity | No cross reactivity with other proteins. |
| Immunogen | AP-2 adaptor polypeptides from bovine brain. |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a |
| Purification | Ascites |
| Storage | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| UniProt | D3ZUY8 |
| NCBI Gene ID | Human(160), Mouse(11771), Rat(308578) |
Available variants
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μg/vial |
Key Facts
Product type
Primary Antibody›
Target: AP-2 complex subunit alpha
| Target | AP-2 complex subunit alpha |
| Also known as | ADTAA; CLAPA1; AP2-ALPHA |
| Concentration | Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml. |
| Species reactivity | Human,Mouse,Rat |
| Observed band | 108 kDa |
| Purity | No cross reactivity with other proteins. |
| Form / buffer | Lyophilized |
| Shelf life | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| Host species | Mouse |
| Clonality | Monoclonal |
| Clone number | Clone: 100/2 |
| Isotype | Mouse IgG2a |
| Conjugation | Unconjugated |
| Non-cross-reactive with | No cross-reactivity with other proteins |
| Tested applications |
WB |
| Dilution range | Western blot, 0.5-1μg/ml, Human, mouse, rat |
| Immunogen | AP-2 adaptor polypeptides from bovine brain. |
| Purification | Ascites |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Human | ||||||||
| Mouse | ||||||||
| Rat |
Specifications
Target
AP-2 complex subunit alpha
Also known as
ADTAA; CLAPA1; AP2-ALPHA
Concentration
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: 100/2
Isotype
Mouse IgG2a
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WB
Immunogen
AP-2 adaptor polypeptides from bovine brain.
Observed band
108 kDa
Dilution range
Western blot, 0.5-1μg/ml, Human, mouse, rat
Purification
Ascites
Storage & Stability
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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