Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-27099

Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2)

ABMIUM Verified™|Collaborator Tested|Boster Bio

Primary Antibody supplied by Boster Bio Read more ›

Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2) Boster Bio
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Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2) Boster Bio

Product Description

Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2) is a research-use antibody from Boster Bio for studying AP-2 complex subunit alpha in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2) catalog # MA1105. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1 (HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al. (2001) identified 3 HIP1-associated proteins, clathrin heavy chain (CLTC)and alpha-adaptin A and C. Coat proteins of approximately 100-kD (adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.

Product information

Key Facts

Product typePrimary Antibodies Target: AP-2 complex subunit alpha
Collaborator Boster Bio
Partner model MA1105
Target AP-2 complex subunit alpha
Reactivity Human,Mouse,Rat
Applications WB
ConjugationUnconjugated
Cross-reactivityNo cross-reactivity with other proteins
Dilution rangeWestern blot, 0.5-1μg/ml, Human, mouse, rat<br>
Host Mouse
Clonality Monoclonal
Clone Clone: 100/2
Isotype Mouse IgG2a
Immunogen AP-2 adaptor polypeptides from bovine brain.
Concentration Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Purification Ascites
Purity No cross reactivity with other proteins.
Shelf life Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
AvailabilityAvailable
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human
Mouse
Rat

Specifications

Target
AP-2 complex subunit alpha
Also known as
ADTAA; CLAPA1; AP2-ALPHA
Concentration
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: 100/2
Isotype
Mouse IgG2a
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WB
Immunogen
AP-2 adaptor polypeptides from bovine brain.
Observed band
108 kDa
Dilution range
Western blot, 0.5-1μg/ml, Human, mouse, rat
Purification
Ascites

Storage & Stability

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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